| 摘要: |
| 【目的】用SSR和SRAP分子标记技术，分析苹果（Malus domestica Borkh.）重要栽培品种的亲缘关系，为苹果杂交育种亲本及组合的选配提供参考。【方法】用亲缘关系较近的金冠和秦冠筛选SSR和SRAP多态性引物，利用SSR 和SRAP分子标记技术对37个苹果栽培品种进行遗传多样性和亲缘关系分析。【结果】筛选出了用于37个苹果主栽品种亲缘关系分析的10对SSR和10对SRAP引物，其分别产生56 和64 条扩增带, 平均多态性比率分别为61.09%和70.8%。根据SSR+SRAP分析结果, 37个苹果品种被聚为6大类。【结论】所选取的引物能够有效地揭示供试苹果品种的遗传多样性，并且苹果品种分类与传统系谱基本一致。 |
| 关键词: 苹果 SSR SRAP 遗传多样性 亲缘关系 |
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| 基金项目:国家现代农业技术体系专项（nycytx-08-01-03）；陕西省“13115”重大科技专项（2010ZDKG-69） |
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| Genetic relationship analysis of apple cultivars with SSR and SRAP markers |
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| Abstract: |
| 【Objective】The study was done in order to select the proper parents in apple breeding.【Method】The genetic diversity and genetic relationship of 37 apple cultivars were studied with the SSR and SRAP markers.【Result】The results showed that 56 and 64 bands were obtained by SSR and SRAP markers amplified through 10 selected primers respectively.Polymorphic percentage was 61.09% and 70.8%.【Conclusion】Based on the SSR+SRAP results,the selected primers were effective in revealing genetic diversity of the tested varieties,and the apple cultivars could be classified into the six groups,which were similar to the traditional classification. |
| Key words: apple SSR SRAP genetic diversity genetic relationship |
