| 摘要: |
| 【目的】对肉鸡群中常见的呼吸道病原体共感染情况进行调查，为肉鸡呼吸道疾病的防控提供流行病学资料。【方法】从山东省济南、潍坊、青岛等地区采集421份有呼吸道症状的病（死）鸡肺脏及160份临床健康肉鸡的肺脏作为供检样品，用特异性核酸探针技术，对其进行H9N2亚型禽流感病毒（H9N2 AIV）、新城疫病毒（NDV）、传染性支气管炎病毒（IBV）、禽偏肺病毒（aMPV）、禽败血性支原体（MG）、鼻气管鸟杆菌（ORT）和大肠杆菌（E.coli）共感染情况的调查。【结果】421份发病肉鸡肺脏中，H9N2 AIV、NDV、IBV、aMPV、MG、ORT和E.coli的检出率分别为53.68%，68.41%，33.49%，54.87%，57.25%，9.02%和41.17%；且存在严重的共感染现象，共感染率高达90.26%，单一感染和阴性个体仅占7.36%和2.38%；发病肉鸡群中，7种呼吸道病原体2重、3重、4重、5重、6重、7重感染的检出率分别达19.48%，26.12%，27.32%，11.88%，4.51%和0.95%，以2重、3重和4重感染较为常见。而临床健康肉鸡肺脏中，H9N2 AIV、NDV、IBV、aMPV、MG、ORT和E.coli的检出率分别为12.50%，23.13%，5.63%，36.87%，21.88%，0%和16.88%；呈阴性和单一感染的样品分别占25.00%和43.75%；多重感染样品仅占31.25%，且主要为2重感染。7种病原体中，H9N2 AIV、NDV、MG和E.coli在肉鸡呼吸道疾病的共感染中起重要作用。【结论】发病肉鸡和临床健康肉鸡均存在混合感染，但以发病鸡群更为严重和复杂；呼吸道病原体多重感染是导致肉鸡呼吸道疾病日益严重的重要原因。 |
| 关键词: 肉鸡 呼吸道病原体 共感染 斑点杂交 |
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| 基金项目:山东省科技发展计划项目（2010GNC10912） |
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| Epidemic investigation of the co-infection of avian respiratory pathogens from broiler in lung |
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| Abstract: |
| 【Objective】Epidemic Investigation of the Co-infection of avian respiratory pathogens from broiler in lung was performed.【Method】421 lung samples of broilers with respiratory symptoms and 160 lung samples of clinically healthy broilers were collected from broiler farms at Jinan,Weifang,and Qingdao in Shandong Province,and then dot blot hybridization was used to detect Avian influenza virus(AIV),Newcastle disease virus(NDV),Infectious bronchitis virus(IBV),Avian pneumovirus viruses(aMPV),Escherichia coli(E.coli),Ornithobacterium rhinotracheale(ORT),and Mycoplasma gallisepticum(MG).【Result】 The results show that the detection rates of the seven pathogens of the 421 lung samples of broilers with respiratory symptoms are 53.68%(H9N2 AIV),68.41%(NDV),33.49%(IBV),54.87%(aMPV),57.25% (MG),9.02%(ORT),41.17%(E.coli) and the co-infection is very serious.The ratio of co-infection is up to 90.26% and single infection and negative is 7.36% and 2.38% respectively.The detection rate of dual infection,triple infection,quadruple infection,quintuple infection,sextuple infection and sevenfold infection is 19.48%,26.12%,27.32%,11.88%,4.51%,and 0.95% respectively,and among which the detection rate of the dual infection,triple infection,quadruple infection is higher than the others.The results show that the detection rate of the seven pathogens of 160 lung samples of clinically healthy broilers is 12.50% (H9N2 AIV),23.13% (NDV),5.63% (IBV),36.87% (aMPV),21.88% (MG),0%(ORT),16.88% (E.coli) and single infection and negative are 43.75% and 25.00% respectively,the ratio of co-infection is only 31.25%.In the seven pathogens,H9N2 AIV,NDV,MG,and E.coli play an important role in the incidence of respiratory disease in broiler.【Conclusion】 The co-infection of the respiratory pathogen of broiler is universal,but the co-infection is more serious in broilers with respiratory symptoms.The co-infection of the respiratory pathogen of broiler is one of the most important epidemiologic factors of the rising incidence in respiratory disease. |
| Key words: broiler respiratory pathogen co-infection dot blot hybridization |
