| 摘要: |
| 【目的】初步探索矮牵牛雄性不育性状的遗传规律，选育雄性不育两用系。【方法】从矮牵牛328自然群体后代中发现并选择不育株，以不育株(08A328)为母本，以7份不同来源的材料为父本进行杂交，杂交后代（F1）自交获得F2，选择F2分离出的不育株与F1回交，同时对F2不育株进行人工自交结实率试验和花粉生活力测定。在F2中优选不育株与经济性状优良的父本进行回交转育，转育后代自交，子代兄妹交，筛选可育株与不育株数量按1∶1分离的株系。【结果】F1后代全可育，F2后代可育株与不育株数量按15∶1分离，回交后代可育株与不育株数量按3∶1分离。不育株花药干瘪微黄，花粉无活力，自交不结实。回交转育后代均可育，自交后可育株与不育株数量按15∶1分离。经兄妹交筛选，初步育成矮牵牛雄性不育“两用系”，其基因型为ms1ms1ms2ms2、MS1ms1ms2ms2或ms1ms1MS2ms2。【结论】该矮牵牛雄性不育性由2对隐性等位核基因控制，不育株率稳定在50%，不育度高。 |
| 关键词: 矮牵牛 雄性不育 遗传 |
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| 基金项目:沈阳市科技攻关项目（1053100-3-02） |
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| Inheritance of male sterility in Petunia hybrida Vilm. |
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| Abstract: |
| 【Objective】The study was conducted to find the inheritance of male sterility in Petunia hybridaVilm.and obtain male sterility A/B lines.【Method】Sterile plants were found and chosen from the 328 natural group in P.hybrida Vilm..Crossing with 7 male parents from different colors and sources,sterile (08A328) was used as female parent F1 hybrids selfing.And the chosen sterile in F2 back crossed.Meanwhile,another staining and testing of self-fruitful ratio of sterile(F2) were carried out.The selected F2 sterile back crossed with high quality male parents.The progeny selfing,and then a sibmating in the self progeny were implemented.【Result】F1:All fertile.F2:15∶1 isolated,Fertile to sterile.Backcross progeny:isolated for 3∶1.A lack of viability in the pollen and light yellowed and withered anthers were showed in sterile plants.The backcross(high quality male parents) progeny was all fertile.Fertile to sterile demonstrated a isolation of 15∶1 after the backcross progeny selfing.Male sterility A/B lines preliminary formed and the genotypes were:ms1ms1ms2ms2,MS1ms1ms2ms2 or ms1ms1MS2ms2.【Conclusion】The male sterility maintained high and stable (50%) and it was determined by 2 recessive nuclear alleles in P.hybrida Vilm.. |
| Key words: Petunia hybrida Vilm. male sterility inheritance |
