| 摘要: |
| 【目的】评价植物源病毒抑制剂VFB的抗烟草花叶病毒(TMV)活性，并初步探讨其作用机理。【方法】以心叶烟(Nicotiana glutinosa L.)和普通烟(Nicotiana Tabacum L.)为寄主材料，采用半叶枯斑法测定了VFB对烟草花叶病毒(Tobacco mosaic virus, TMV) 的体外钝化作用及对TMV初侵染和增殖的抑制作用，并用透射电镜技术研究了VFB对感染TMV烟草叶片超微结构的影响。【结果】VFB对TMV具有明显的体外钝化作用，在对TMV钝化0.5，1和2 h后，其抑制率分别为44.41%，55.62%和64.43%；VFB对TMV侵染心叶烟具有较好的预防作用，其50倍稀释液在24和48 h的抑制率分别达到54.33%和55.60%，均优于对照药剂病毒A；VFB对TMV有一定的治疗作用，其50倍稀释液的抑制率为36.15%，略低于对照药剂病毒A。烟草叶片病理结构的超微观察表明，普通烟感染TMV后，叶片中大部分细胞结构会受到不同程度的破坏，经VFB处理的烟草，花叶症状减轻，叶片中的TMV粒子显著减少，叶绿体和线粒体的解体症状减轻，并产生大量嗜锇颗粒和膜体。【结论】VFB对TMV具有较好的抑制作用。 |
| 关键词: 植物源农药 VFB 烟草花叶病毒 诱导抗性 组织病理学 |
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| 基金项目:农业部公益性行业科研专项（200903052）；西北农林科技大学青年学术骨干支持计划项目 |
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| Anti-tobacco mosaic virus activity of VFB and its effect on ultrastructure of Nicotiana tabacum |
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| Abstract: |
| 【Objective】The experiment was conducted to evaluate anti-TMV activity of VFB and explore its mechanism.【Method】Taking Nicotiana glutinosa and Nicotiana tabacum as the host material,the anti-TMV activity of VFB in vitro was tested by local lesion and histopathologic changes of leaves of N.tabacum were observed by TEM.【Result】The results showed that VFB displayed significant inactivation,the inhibition rate of necrotic local lesions was 44.41%, 55.62%,64.43% after mixing VFB with TMV for 0.5,1 and 2 h.VFB had a good preventive effect on TMV infection to N.glutinosa,the preventive effect reached 54.33% (24 h) and 55.60% (48 h) treated with VFB water solution for 50 times.VFB also had a therapeutic effect on TMV,the therapeutic efficacy was 36.15% treated with VFB water solution for 50 times.The results of electron microscopic observation showed the ultrastructure of leaves of N.tabacum infected by TMV was altered partly.After VFB treatment,TMV particle decreased significantly,the disorganization of chloroplast and mitochondrion was mitigated,and VFB induced tobacco to produce a great deal of osmiumphilic bodies.【Conclusion】The VFB had the ability of inactivating TMV. |
| Key words: botanical pesticide VFB tobacco mosaic virus induced resistance histopathology |
