| 摘要: |
| 【目的】研究L-肉碱强化卤虫对鲤鱼开口苗脂肪酸组成和C/N比率的影响,为鲤鱼开口苗的饲养提供参考。【方法】分别用质量浓度为0(对照)，1，100，1 000 mg/L的L-肉碱强化卤虫无节幼体（Artemia sp.）12和24 h，然后投喂给鲤鱼(Cyprinus carpio)开口苗，21 d后测定鱼苗的脂肪酸组成和C/N比率。【结果】投喂L-肉碱强化12 h的卤虫时，1 mg/L L-肉碱处理组鲤鱼开口苗的∑C14-24脂肪酸含量、饱和脂肪酸含量和单不饱和脂肪酸含量较对照组显著降低（P＜0.05）；1 000 mg/L L-肉碱处理组鲤鱼开口苗的单不饱和脂肪酸含量较对照组显著升高（P＜0.05）；L-肉碱对鲤鱼开口苗的多不饱和脂肪酸含量、∑n-3+∑n-6和∑DHA+EPA含量均无显著影响（P＞0.05）；1 000 mg/L L-肉碱处理组鲤鱼开口苗的C/N比率显著低于对照组和1 mg/L L-肉碱处理组（P＜0.05）。投喂L-肉碱强化24 h的卤虫时，1和100 mg/L L-肉碱处理组鲤鱼开口苗的∑C14-24脂肪酸和饱和脂肪酸含量较对照组显著降低（P＜0.05），但2组之间差异不显著（P＞0.05）；1 000 mg/L L-肉碱处理组鲤鱼开口苗的∑C14-24脂肪酸和饱和脂肪酸含量较对照组显著升高（P＜0.05）；1 mg/L L-肉碱处理组鲤鱼开口苗的单不饱和脂肪酸含量较其他3组显著降低（P＜0.05），多不饱和脂肪酸、∑n-3+∑n-6和∑DHA+EPA含量与对照组差异不显著（P＞0.05）；100 mg/L和1 000 mg/L L-肉碱处理组鲤鱼开口苗的多不饱和脂肪酸、∑n-3+∑n-6和∑DHA+EPA A含量较对照组显著升高（P＜0.05），且2组之间差异显著（P＜0.05）；1，100 mg/L L-肉碱处理组鲤鱼开口苗的C/N比率较对照组和1 000 mg/L L-肉碱处理组显著降低（P＜0.05）。【结论】本试验条件下，以质量浓度100 mg/L L-肉碱强化卤虫24 h后再投喂鲤鱼开口苗，可显著改善鲤鱼开口苗的脂肪酸组成和C/N比率。 |
| 关键词: L-肉碱 强化卤虫 鲤鱼 开口苗 脂肪酸组成 C/N比率 |
| DOI： |
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| 基金项目:国家自然科学基金项目(30671621) |
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| L-carnitine enriched Artemia on fatty acids composition and C/N ratio in first feeding larvae of common carp,Cyprinus carpio |
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CHEN Yuke1, LIN Lili2, SONG Dan,et al1
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1.Faculty of Animal Science and Technology, Jilin Agricultural University;2.Faculty of Animal Science and Technology ,Jilin Agricultural University
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| Abstract: |
| 【Objective】Effects of L-carnitine enriched Artemia on fatty acids composition and C/N ratio in first feeding larvae of freshwater common carp (Cyprinus carpio) were studied to offer basis to improve culturing technology of the larvae.【Method】The experimental larvae were reared in 50 L tank with stocking density of 40 fish/L at (25±1) ℃ for 21 days.The newly hatched Artemia nauplii,enriched with 4 levels (0,1,100,1 000 mg/L) of L-carnitine at (25±1) ℃for 12 h and 24 h respectively,were used as starter food for rearing larvae.【Result】In the 12 h-enriched groups,∑C14-24 FA,SFA and MUFA contents were significantly(P<0.05) lower by feeding 1 mg/L L-carnitine enriched nauplii than those of the control group;MUFA content was improved significantly (P<0.05) by feeding 1 000 mg/L L-carnitine enriched nauplii than the control group,no significant differences (P>0.05) were observed on PUFA,∑n-3+∑n-6 and ∑DHA+EPA content in the treatment groups;C/N ratio was decreased significantly (P<0.05) by feeding 1 000 mg/L L-carnitine enriched nauplii than 1 mg/L L-carnitine enriching group and the control(P<0.05).In the 24 h enriched groups,∑C14-24 FA and SFA contents were significantly(P<0.05) lower in 1 and 100 mg/L L-carnitine enriching groups than those of the control group,but no significant differences (P>0.05) were observed between them;∑C14-24 FA and SFA contents were improved significantly(P<0.05) by feeding 1 000 mg/L L-carnitine enriched nauplii than the control group;MUFA content of larvae was decreased significantly (P<0.05) by feeding 1 mg/L L-carnitine enriched nauplii than that of the other groups;no significant differences (P>0.05) were observed on PUFA,∑n-3+∑n-6 and ∑DHA+EPA content between 1 mg/L L-carnitine enriching groups and the control;There was significantly (P<0.05) higher PUFA,∑n-3+∑n-6 and ∑DHA+EPA content for larvae in 100 and 1 000 mg/L L-carnitine enriching groups than in the control,but significant differences (P>0.05) were observed between them;The C/N ratio of larvae was significantly(P<0.05) lower by feeding 1 and 100 mg/L L-carnitine enriched nauplii than that of the control and 1 000 mg/L L-carnitine enriching groups.【Conclusion】The fatty acids composition and C/N ratio of larvae were improved by feeding 100 mg/L L-carnitine enriched nauplii in the 24 h enriched groups. |
| Key words: L-carnitine enriched Artemia common carp(Cyprinus carpio) first feeding larvae fatty acids composition C/N ratio |
