| 摘要: |
| 【目的】了解猪细小病毒(PPV)感染对抗病毒相关细胞因子mRNA转录时相的影响，探讨宿主-病毒之间的作用关系。【方法】对PPV感染PK-15细胞的病变进行了显微观察，运用荧光定量PCR技术，测定和分析PPV感染PK-15细胞后，细胞病毒DNA含量及巨噬细胞炎症蛋白1α（MIP-1α）、转化生长因子β1（TGF-β1）、磷酸甘油酸激酶1（PGK1）和MURR1等细胞因子mRNA相对表达量的变化。【结果】PPV可以快速诱导PK-15细胞产生细胞病变。感染后1 h即可检测到PPV DNA，随着时间的延长，PPV DNA 含量逐渐增加，并于48 h时达到峰值，相对含量为1 800左右。MIP-1α和PGK1 mRNA的相对表达量在感染后2 h显著增加，之后下降；TGF-β1 mRNA的相对表达量在72 h时显著增加；MURR1 mRNA的相对表达量在24 h时显著增加，48 h后下降。【结论】PPV感染可引起PK-15细胞抗病毒因子mRNA表达水平升高。 |
| 关键词: 猪细小病毒 细胞因子 PK-15细胞 转录时相 |
| DOI： |
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| 基金项目:河南省杰出人才创新基金项目(0621002100) |
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| Transcriptional profiles of four kinds of antivirus cytokines mRNA on PK-15 cell cultures following infection with Porcine parvovirus |
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GENG Jingwei1, LI Houwei2, YIN Haiyan,et al3
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1.Key Laboratory for Animal-derived Food Safety of Henan Province;2.College of Animal Science and Veterinary Medicine ,Henan Agricultural University;3.Henan Province Academy of Agricultural Quality Standards and Testing Technology Research Center
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| Abstract: |
| 【Objective】The study was done to survey the transcriptional profiles of cytokines,particularly antivirus cytokines mRNA transcriptional profiles of Porcine parvovirus infection,and the host PPV interaction.【Method】We used PPV to infect the Porcine Kidney 15 cells,observed the PK-15 cells of inoculation PPV by microscope,measured and analyzed the viral DNA using real-time PCR.We also detected the transcript levels of cytokines MIP-1α,TGF-β1,PGK1 and MURR1 by real-time PCR.【Result】PPV can induce cytopathic effect in PK-15 cells.The cells can be detected with PPV on 1 h postinoculation(p.i.),and the relative content of PPV DNA achieved 1 800 on 48 h p.i..We found that the transcript levels of MIP-1α and PGK1 increased obviously on 2 h p.i.,then decreased,the transcript level of TGF-β1 increased obviously on 72 h p.i.,the transcript level of MURR1 increased obviously on 24 h p.i.,then decreased after 48 h p.i..【Conclusion】The levels of antiviral cytokines increased when PPV infected PK-15 cell. |
| Key words: Porcine parvovirus cytokines PK-15 cell transcriptional profiles |
