| 摘要: |
| 【目的】研究野猪源与家猪源猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus, PRRSV)陕西分离株的遗传变异情况及分子生物学特征。【方法】根据VR-2332和HB-1(sh)/2002毒株序列设计引物，对2株PRRSV陕西分离毒株Shaanxi-1(家猪源)、Shaanxi-2(野猪源)全基因分段进行扩增并测序，将测序结果依次拼接得到病毒的全基因，然后进行序列的比对分析。【结果】PRRSV Shaanxi-1、Shaanxi-2毒株高度同源且均属于美洲型毒株，它们与2006年以前分离的毒株相比存在明显差异（与BJ-4株的核苷酸同源性仅为89%），而与高致病性PRRSV（HP-PRRSV）保持较高的同源性（相似率为99%左右）。Shaanxi-1、Shaanxi 2分别呈现不同的变异特点，Shaanxi-1 ORF3、ORF5基因变异较大，ORF6、ORF7较为保守；Shaanxi-2 ORF7基因变异最大，ORF3～ORF6较为保守。分析发现，Shaanxi-1、Shaanxi-2具有HP-PRRSV的分子特征，但部分位点氨基酸的突变明显区别于HP-PRRSV。【结论】不同来源的Shaanxi-1、Shaanxi-2毒株均属于HP-PRRSV演化中的一个分支，但其发生了部分变异，使其具备了新的特点。 |
| 关键词: 猪繁殖与呼吸综合征病毒 野猪与家猪 全基因测序 遗传进化分析 |
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| 基金项目:陕西省“13115”科技创新工程重大科技专项(2007ZDKG-13) |
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| Genomic sequencing and phylogenetic analysis of PRRSV isolated from domestic pig and wild boar in Shaanxi Province |
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| Abstract: |
| 【Objective】The research was done to study genetic variation and molecular biological characteristics of PRRSV with different sources isolated from Shaanxi Province.【Method】Primers were designed according to VR-2332 and HB-1(sh)/2002 strains,genome of two PRRSV strains Shaanxi-1(source of domestic pig),Shaanxi-2(source of wild boar) isolated from Shaanxi Province was amplified and then sequenced,whole genome sequence was obtained by splicing of correct sequencing and was aligned with other strains.【Result】The results showed that Shaanxi-1 had a high homology with Shaanxi-2 and both of them belonged to American type strain.Even though they had significant differences from the strains isolated before 2006 (the homology only 89% compared with BJ-4),they shared close to 99% homology with HP-PRRSV.However,Shaanxi-1,Shaanxi-2 had different variation characteristics,Shaanxi-1 ORF3,ORF5 varied greatly,ORF6,ORF7 revealed more conservative;Shaanxi-2 ORF7 got more variation relatively,ORF3～ORF6 seemed more conservative.Analysis showed that Shaanxi-1,Shaanxi-2 had common molecular characteristics with HP-PRRSV,but mutations of a few amino acids different from HP-PRRSV also existed.【Conclusion】In summary,we could conclude that both Shaanxi-1,Shaanxi-2 from different sources are a branch of HP-PRRSV,however,part of the gene mutations enable them possess new features. |
| Key words: PRRSV domestic pig and wild boar genomic sequencing phylogenetic analysis |
