| 摘要: |
| 【目的】建立一种快速、高效、准确的动物源性食品材质的鉴定方法。【方法】采用PCR方法扩增猪、羊、牛动物组织mtDNA的COⅠ基因，对PCR扩增片段进行测序并构建系统发生树；以Hinf Ⅰ酶为内切酶，对3种样品的COⅠ基因进行聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)分析。【结果】对测序结果进行多重序列比对发现，猪、羊、牛COⅠ基因的碱基差异为19.0%～25.9%，物种内的碱基差异仅为0.1%～1.6%。猪、羊、牛COⅠ因PCR扩增片段经HinfⅠ酶切后的长度依次为40，411，449 bp；144，376，381 bp和46，377，479 bp，限制性片段长度具有物种特异性。【结论】COⅠ基因的序列及PCR-RFLP分析，可用于猪、牛、羊动物源性食品的材质鉴定。 |
| 关键词: 动物性食品 材质鉴定 PCR扩增 COⅠ基因 |
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| 基金项目:北京市科学技术研究院创新团队项目(IG200905N) |
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| Identification of molecular biology to identify three kinds of animal flesh |
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| Abstract: |
| 【Objective】This article aims to establish an effective,rapid and accurate method to identify animal-derived food.【Method】COⅠ mtDNA sequences from pork,mutton and beef were amplified by the polymerase chain reaction (PCR) technique.The PCR-products was cleaved by HinfⅠrestriction enzymes.【Result】The study resulted in 40,411 and 449 bp (pork);144,376 and 381 bp (mutton);46,377 and 479 bp (beef) fragments.A diversity was demonstrated in pork,beef and mutton.Multiple Sequence Alignment result shows that nucleotide difference between swines,ovis aries and bovine’s COⅠ gene is 19.0% to 25.9%,nucleotide difference within species is only 0.1% to 1.6%.【Conclusion】The result shows that pork,mutton and beef can be identified by using PCR-RFLP analysis of the COⅠ gene in animal flesh. |
| Key words: animal flesh identification PCR COⅠgene |
