| 摘要: |
| 【目的】筛选适宜沙枣多酚纯化的大孔树脂。【方法】通过静态吸附与解析试验，从AB-8、NKA-9、NKA、D4020、X-5和D101中筛选可用于沙枣多酚纯化的大孔树脂，并研究其对沙枣多酚的动态吸附和解析性能。【结果】NKA-9树脂对沙枣多酚的饱和吸附量为19.58 mg/g，吸附等温线符合Langmuirh和Freundlich方程，动力学曲线符合Langmuir方程，饱和吸附时间为7 h左右；NKA-9大孔树脂纯化沙枣多酚的最佳工艺条件为：上柱液pH 4，上柱液中沙枣多酚质量浓度1.00 mg/mL，上样液体积25.12 mL，上样流速1.0 mL/min，洗脱液乙醇体积分数60%，洗脱剂体积18.84 mL，洗脱剂流速1.0 mL/min。【结论】NKA-9型大孔树脂表现出较好的吸附性能与解析效果，能很好地富集纯化沙枣多酚。 |
| 关键词: 沙枣 多酚 大孔吸附树脂 纯化 |
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| 基金项目:兰州理工大学优秀青年教师培养计划资助项目（0908ZXC164） |
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| Dynamic absorption and desorption of polyphenols from Elaeagnus angustifolia L.by macroporous absorbent resins |
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| Abstract: |
| 【Objective】This study was to study a suitable resin for purifying Elaeagnus angustifolia L. polyphenol.【Method】Dynamic absorption and desorption experiments were carried out to select suitable resin from AB-8,NKA-9,NKA,D4020,X-5 and D101 of macroporous absorbent resin for E.angustifolia L.polyphenol．【Result】The static saturated adsorption capacity of NKA-9 resin was 19.58 mg/g while the time for it to achieve saturation adsorption was 7 h.Besides,the absorption isotherm data of NKA-9 resin could well fit the Langmuir equation and Freundlich equation,and the absorption kinetic also fitted the Langmuir equation;The optimum absorption conditions of macroporous resin NKA-9 were pH value 4,concentration of feed 1.00 mg/mL,volume of polyphenol sample 25.12 mL,feeding rate 1.0 mL/min.And the the optimum desorption conditions were elute ethanol concentration 60%,elute volume 18.84 mL,elution rate 1.0 mL/min.【Conclusion】 Macroporous resin NKA-9 possessed the strongest absorbend ability and easier desorption property,which is a better macroporous absorbent resin for purificating polyphenols in E.angustifolia L.． |
| Key words: Elaeagnus angustifolia L. polyphenol macroporous absorbent resin purification |
