| 摘要: |
| 【目的】用SSR标记分析柿属植物种质资源的遗传多样性，为其有效利用提供依据。【方法】以7个柿种或变种的48份材料为研究对象，利用30对SSR标记对其进行遗传多样性分析。采用NTSYS-pc 2.10e分析软件对SSR数据进行聚类分析。【结果】共检测到 83个等位基因，每对 SSR引物检测到 3～6个等位基因变异，平均为 4.9个。供试柿品种间的遗传相似系数介于0.397～1.000，平均为 0.698，范围较大，说明柿资源种类较多、来源较广泛、遗传多样性较丰富。48份柿材料在相似系数为0.66水平上分为4大类，第Ⅰ类包括浙江柿和5个美洲柿及1个柿品种；第Ⅱ类只有2个资源，即油柿和野柿；第Ⅲ类为柿种下的大部分柿品种（27个）；第Ⅳ类包括2个有核君迁子、3个无核君迁子、2个野柿、3个金枣柿及2个柿种下的品种；不同种类的柿种质资源基本分别聚在一起。同一种类下的不同类型也能区分开来。【结论】SSR标记是研究柿种质资源遗传多样性的有效手段。 |
| 关键词: 柿属植物 种质资源 SSR标记 遗传多样性 |
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| 基金项目:国家科技基础条件平台工作子项目（2005DKA21002-22） |
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| Analysis of genetic diversity of Diospyros spp germplasm resources by using SSR markers |
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| Abstract: |
| 【Objective】The study was to analyze the genetic diversity of Diospyros spp germpleasm resources by using SSR markers to provide basis for their effective application.【Method】48 different accessions of seven species or varieties,and 30 pairs of SSR primers were selected to assess their genetic diversity and relatedness by analyzing them,and cluster analysis was done using NTsys pc2.10e.【Result】A total of 83 alleles were detected with a mean value of 4.9 alleles locus (range 3-6).Varieties of persimmon genetic similarity coefficient ranged between 0.397-1.000,averaging 0.698,with larger range,which shows more types of persimmons,broader range of sources,and richer genetic diversity.48 Persimmon resources could be classified into 4 groups.GroupsⅠinclude a D.glaucifolia,five D.vaginiana and one D.kaki.GroupⅡ just has two accessions:a D.olifera and a D.kaki.var.most persimmon varieties (27)are in group Ⅲ.GroupsⅣ include 2 D.lotus with seeds,3seedless D.lotus,2 D.kaki.var.3 Jinzaoshi and 2 varieties of D.kaki.Different species can be clustered respectively and different types in intra-species can be discriminated by SSR markers,indicating that it is feasible to use SSR markers to distinguish persimmon germplasm.【Conclusion】Using SSR markers is an available methods to study the genetic diversity of persimmon germplasm. |
| Key words: Diospyros spp germplasm resources SSR markers genetic diversity |
