| 摘要: |
| 【目的】研究陕西土荆芥挥发油的化学成分及对15个临床消毒常见菌株的抗菌性能，为土荆芥天然消毒杀菌产品的开发利用提供科学依据。【方法】采用水蒸汽蒸馏法提取土荆芥挥发油，GC-MS测定其化学成分，分别用连续2倍梯度液体稀释法、液体转染法测定最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。【结果】从陕西土荆芥挥发油中共检测到16种化学成分，并鉴定出了其中的15种成分，主要化学成分有p-伞花烃（29.95%）、驱蛔素（22.97%）、冰片烯（20.74%）、α-松油烯（17.67%）；在临床消毒常见的15种菌种中，土荆芥挥发油除对绿脓假单胞菌CMCC10211株无抑制作用外，对炭疽芽胞杆菌CMCC63001、白假丝酵母菌CMCC850216、乙型溶血性链球菌CMCC32210等其他14株标准菌株均有较好的抑制作用。【结论】陕西土荆芥挥发油的主要化学成分为p-伞花烃、驱蛔素、冰片烯和α-松油烯；土荆芥挥发油具有广谱抗菌作用，对临床消毒常见的致病菌有较强的抑制和灭活作用。 |
| 关键词: 土荆芥 挥发油 抗菌活性 |
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| 基金项目:国家“十一五”科技支撑计划项目（2008BAD98B08）；中国科学院知识创新项目(KZCX2-XB2-05-01) |
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| Chemical constituents and antibacterial activities of essential oils from Chenopodium ambrosioides L. |
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| Abstract: |
| 【Objective】The chemical constituents and antibacterial activities of volatile oil from Chenopodium ambrosioides L (CML)were studied for its exploitation.【Method】The volatile oil was extracted by steam distillation from CML,its chemical constituents were identified by GC MS,meanwhile the antibacterial activities were expressed by minimal inhibitory concentration(MIC) and minimum bactericidal concentration (MBC) and tested by continuous double dilution method and liquid infection protocol methods,respectively.【Result】Sixteen components were detected and fifteen components were identified.The main chemical constituents of volatile oil from CML were p-cymene (29.95%),Ascaridole (22.97%),Bornylene (20.74%),and α-terpinene (17.67%).The volatile oil exhibited significant antibacterial activities to the common 15 bacterial strains (Bacillus anthracis CMCC63001,Candida albicans CMCC850216 and Streptococcus hemolysis β-CMCC32210 etc) of clinical sterilization except Pseudomonas aeruginosa CMCC10211 strain.【Conclusion】 The main chemical constituents of volatile oil from CML were p-cymene,Ascaridole,Bornylene,and α-terpinene.The volatile oil exhibited significant antibacterial activities. |
| Key words: Chenopodium ambrosioides L. volatile oil antibacterial activity |
