| 摘要: |
| 【目的】研究悬浮培养条件下雷公藤不定根营养成分的消耗动态与不定根生长之间的关系，为雷公藤组织培养生产次生代谢产物中培养基的改良及无机元素的及时补充提供参考。【方法】以NT为基本培养基，将雷公藤不定根悬浮培养，每隔5～10 d对培养基中碳源、氮源、磷酸盐、钾盐、钙盐、镁盐等营养物质的消耗动态及不定根增长量进行测定。【结果】雷公藤不定根对碳、氮、磷酸盐以及钾、钙、镁等营养物质的消耗并不是均匀的。蔗糖、氮、磷酸盐和钾盐在培养后期几乎被耗尽，其中氮的消耗速率最快，在不定根生长进入对数期以前，氮元素已经开始大量消耗，进入对数生长期后，培养基中的氮源90%被消耗。蔗糖、磷酸盐、钾盐和钙盐的消耗表现为先快后慢，早期吸收迅速，而在快速生长期其消耗速度减慢。细胞停滞生长以后，蔗糖、氮、磷酸盐和钾盐仍有大量消耗。NT培养基中碳、氮、磷、钾在培养的前期已经不能满足不定根生长的需要。雷公藤不定根培养过程中对钙和镁的消耗相对较少，培养结束后培养基中还有48.3%的钙离子和34.2%的镁离子存在。【结论】NT培养基中，碳源、氮源、磷酸盐和钾盐不足是影响雷公藤不定根生长的主要因素。 |
| 关键词: 雷公藤 组织培养 不定根 营养物质 消耗动态 |
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| 基金项目:公益性行业(农业)科研专项(200903052)；陕西省“13115”科技创新工程重大科技专项（2009ZDKG-01） |
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| Study on kinetic investigation of Tripterygium wilfordii adventitious root growth and nutrient consumption in vitro |
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| Abstract: |
| 【Objective】 The kinetics of nutrient consumption and adventitious root growth were investigated under adventitious root suspension cultures of Tripterygium wilfordii,to lay the foundation for the medium improvement of selective medium and supplied inorganic elements of production on the secondary metabolites.【Method】 Take NT as the basic medium,while in the suspension culture process,a determination on the nutrient consumption,such as carbon source,nitrogen source,PO43-,K+,Ca2+,Mg2+ and the adventitious root growth was needed in each 5-10 d.【Result】 The consumption of the T.wilfordii adventitious root on the carbon source,nitrogen,PO43- as well as K+,Ca2+ and Mg2+ was not uniform.Sucrose,nitrogen,PO43- and K+ were almost used up in the latter developing period,in which nitrogen was the fastest.Before entering the logarithmic phase,the nitrogen had already begun to consume;90% of it could be consumed in the logarithmic phase.The consumption of sucrose,PO43-,K+ and Ca2+ was quick at first,and slow later.The early absorption was rapid,while in the fast growing period,consumption slowed down.The sucrose,nitrogen source,PO43- and K+ still had the mass consumptions after the stagnation of the adventitious roots growth.In the NT medium,the carbon source,nitrogen,PO43- and K+ already could not meet the needs of the growth of the adventitious root in the early culture period.In the culture process of the adventitious root,the consumption of Ca2+ and Mg2+ was relatively few.After the culturing,there were still 48.3% Ca2+ and 34.2% Mg2+ existing in the medium.【Conclusion】 In the NT medium,the insufficiency of the carbon source,nitrogen source,PO43- and K+ were the main factors affecting the growth of the T.wilfordii adventitious root. |
| Key words: Tripterygium wilfordii Hook.f. tissue culture adventitious root nutrient consumption kinetic investigation |
