| 摘要: |
| 【目的】研究硫酸酯化修饰对鸡腿菇多糖（WPC）抗氧化活性的影响，为天然抗氧化剂的开发利用提供理论依据。【方法】采用氯磺酸-吡啶法，制备硫酸酯化多糖（SWPC）；以取代度为指标,采用单因素试验对制备SWPC的酯化条件进行优化；采用邻苯三酚自氧化法和邻二氮菲-金属铁离子H2O2体系，研究SWPC的体外抗氧化活性。【结果】最佳酯化条件为：酯化试剂体积比（V（氯磺酸）∶V（吡啶））1∶3 ,反应温度90 ℃,反应时间2 h。SWPC对羟自由基和超氧阴离子自由基均具有清除能力；与WPC相比，SWPC清除羟自由基的能力显著提高，但清除超氧阴离子自由基的能力有所下降。【结论】SWPC对羟自由基和超氧阴离子自由基均具有一定的清除能力，但对不同基团的敏感性差异明显。 |
| 关键词: 鸡腿菇 多糖 硫酸酯化 抗氧化活性 |
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| 基金项目:国家自然科学基金资助项目（10874108）；陕西省自然科学基础研究计划资助项目（SJ08A16）；陕西师范大学校级大学生创新性实验基金资助项目 |
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| Optimization on the sulfated modification of polysaccharides from Coprinus comatus and evaluation of antioxidant activity |
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| Abstract: |
| 【Objective】 The effects of Sulfated modification on Polysaccharides from Coprinus comatus were studied to provide a basis for natural antioxidants’ development.【Method】 Polysaccharides from C.comatus extracted by hot-water(WPC) was sulfated as the chlorosulfonic acid-Pyridine method.According to the degree of substitution,single factor experiment was operated to optimizing the sulfated modificiation.The effects of sulfated C.comatus polysaccharides(SWPC) on superoxide anion radicals and scavenging hydroxyl radicals were determined by pyrogallol autoxidation and phenanthroline metal iron H2O2 system respectively.【Result】 The result showed that 1∶3 volume ratio of chlorine sulfonic acid and pyridine,90 ℃,2 h were the optimum conditions.The effects on both scavenging hydroxyl radicals and superoxide anion radicals could be shown evidently.Compared with WPC,the effect of SWPC on scavenging hydroxyl radicals had been improved a lot,while diminished on scavenging superoxide anion radicals.【Conclusion】 SWPC can scaveng both hydroxyl radicals and superoxide anion radicals,but the sensitive effects on them are quite different. |
| Key words: Coprinus comatus polysaccharide sulfation antioxidant activity |
