| 摘要: |
| 【目的】通过对春性甘蓝型油菜胚状体成苗影响因素的研究，以提高胚状体成苗效率，缩短DH群体构建的时间。【方法】以小孢子培养获得的5 964个子叶形胚状体为材料，研究了胚状体长度、培养基分装方式、琼脂质量浓度、低温处理时间与胚状体成苗的关系。【结果】胚状体在固体培养基上的放置方式影响其形态发育；长度为3～4 mm的胚状体成苗率最高，达到60.1%；分装方式Ⅲ,即分装20 min后封口，培养基凝固后表面没有积水的培养基出苗最好，成苗率达到65.2%；随琼脂质量浓度的增加，胚状体成苗率呈先上升后下降的趋势，琼脂质量浓度为12 g/L时的成苗率最高，达到61.3%；4 ℃低温处理14 d可使胚状体一次成苗率提高到65.5%。【结论】缺氧和低温处理影响胚状体的形态发育；胚状体发育程度影响成苗率；相对干燥的环境有利于成苗；琼脂质量浓度通过影响培养基中可利用水的分配、培养瓶内湿度以及乙烯浓度影响小孢子胚成苗率；低温可能通过影响胚状体内源激素水平起到提高成苗率的作用。 |
| 关键词: 春性甘蓝性油菜 小孢子培养 胚状体 成苗率 |
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| 基金项目:青海省科技厅项目（2009-Z-702） |
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| Studies on plantlet formation of microspore embryoid in spring Brassica napus L. |
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| Abstract: |
| 【Objective】Factors affecting the plantlet of embryoid were studied in order to enhance plantlet rate of embroids.【Method】Using 5 964 embryoids obtained from microspore culture as material,the paper analyzed differences in the rate of seeding,which were caused by embryoid length,humidity in flask,agar concentration in B5 medium and low temperature processing time.【Result】The way of placement in the solid medium affected embryoids morphological development.The seedling rate of 3-4 mm embryoid was the highest,reaching 61.3%.The seedling rate of the third pecking method was the highest,reaching 65.2%.With the increase of agar ccncentration embryoid seedling rate increased first and then decreased.The medium with 12 g/L agar presented the highest seedling rate 61.3%.4 ℃ low temperature treatment for 14 days obtained a seedling rate of 65.5%.【Conclusion】Hypoxia and low temperature treatment affected morphogenesis development of embryoid.Plantlet was related to the degree of embryoid development,humidity in flask,agar concentration and low temperature treatment time.B5+1.2% agar was a suitable medium.Low temperature treatment at 4 ℃ for 14 days was the best. |
| Key words: spring Brassica napus L. microspore culture embryoid seedling rate |
