| 摘要: |
| 【目的】 研究异叶败酱多糖抑制U14宫颈癌实体瘤小鼠肿瘤生长的作用机制。【方法】 建立小鼠宫颈癌（U14）实体瘤模型，以灌胃生理盐水和皮下注射环磷酰胺(25 mg/kg)分别作为阴性和阳性对照，观察异叶败酱多糖不同剂量（30，60，90 mg/kg）灌胃给药14 d后，对小鼠肿瘤生长抑制作用的影响；检测异叶败酱多糖对荷瘤小鼠血清乳酸脱氢酶（LDH）和碱性磷酸酶（AKP）活性的影响；用原位末端标记（TUNEL）法检测肿瘤细胞的凋亡情况；利用免疫组织化学法分析与细胞凋亡密切相关蛋白（突变型 p53、Bcl-2、Bax）的表达情况。【结果】 与阴性对照组相比，异叶败酱多糖中、高剂量组瘤质量显著下降，荷瘤小鼠血清LDH活性显著降低，Bax蛋白表达量显著升高，突变型p53和Bcl-2蛋白表达量显著下降（P＜0.05）；异叶败酱多糖各组肿瘤组织的细胞凋亡数较阴性对照组均显著提高（P＜0.05），荷瘤小鼠血清AKP活性有所升高，但与阴性对照差异不显著（P＞0.05）。与阳性对照组相比，异叶败酱多糖中、高剂量组抗U14宫颈癌效果接近于临床化疗药环磷酰胺。【结论】 异叶败酱多糖具有抑制实体瘤U14生长的作用，揭示该多糖可能通过调节细胞凋亡相关基因的表达，进而促进肿瘤细胞的凋亡而发挥其抗肿瘤作用。 |
| 关键词: 抗肿瘤活性 异叶败酱多糖 细胞凋亡 宫颈癌小鼠 |
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| 基金项目:河北省秦皇岛科技局转基因动物生产基因工程药物研究项目(D08) |
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| Anti-tumor activity of polysaccharides isolated from Patrinia heterophlla Bunge |
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| Abstract: |
| 【Objective】The aim of our research was to investigate the effect of Patrinia heterophlla Bunge polysaccharide (PHB-P) on U14 bearing mice.【Method】The tumor inhibition ratio was calculated by the model U14 bearing tumor mice.Subcutaneous injection of normal saline and given with cyclophosphamide 25 mg/kg were used as negative and positive controls,treated with PHB-P (30,60,90 mg/kg) for 14 days.Detection of apoptosis was performed by TUNEL staining assays.The activity of serum LDH and AKP was examined using the kits.Imunohistochemical analysis assay was applied on the protein expression productions of apoptosis associated mutant p53,Bcl-2 and Bax genes in tumor tissues.【Result】The results showed that the groups of PHB-P (60,90 mg/kg),compared with the negative control group,significantly decreased tumor weight and the difference was significant (P<0.05);compared with the negative control group,the number of apoptotic cells of the PHB-P (60,90 mg/kg) groups significantly increased (P<0.05);compared with the negative control group,tumor bearing mice serum LDH activity of the groups of PHB-P (60,90 mg/kg) significantly decreased (P<0.05);In each group of PHB P(30,60,90 mg/kg),serum AKP activity increased compared with the negative control group,but the difference was not significant (P>0.05);Bax protein expression was significantly higher than that of the negative control group (P<0.05),mutant p53 and Bcl-2 protein expression in PHB-P (60,90 mg/kg) group compared with the negative control group decreased significantly (P<0.05).Compared with the positive control group,the effects of anti-tumor activity of the PHB-P (60,90 mg/kg) was close to the clinical chemotherapeutic agent cyclophosphamide.【Conclusion】Our data suggested that PHB-P might induce tumor cells apoptosis and inhibit tumor growth,including regulation of the apoptosis associated genes. |
| Key words: anti-tumor activity Patrinia heterophlla Bunge polysaccharides cell apoptosis uterine cervical cancer mice |
