| 摘要: |
| 【目的】探讨酸法与酶法提取葡萄皮渣可溶性膳食纤维的最佳工艺组合，并比较8种酿酒葡萄皮渣中可溶性膳食纤维含量的差异。【方法】（1）用HCl提取葡萄皮渣中的可溶性膳食纤维，以HCl浓度、提取温度、提取时间、料液比4因素设计四因素三水平正交试验，确定酸法提取葡萄皮渣可溶性膳食纤维的最佳工艺条件；（2）以纤维素酶液提取葡萄皮渣中的可溶性膳食纤维，设计四因素三水平正交试验（4因素包括纤维素酶用量、提取温度、提取时间、料液比），确定酶法提取葡萄皮渣中可溶性膳食纤维的最佳工艺条件；（3）采用酸法和酶法获得的最佳工艺条件，比较8种酿酒葡萄皮渣中可溶性膳食纤维的含量。【结果】（1）酸法提取葡萄皮渣可溶性膳食纤维的最佳工艺组合为：HCl浓度0.389 mol/L，提取温度75 ℃，提取时间75 min，料液比1∶20；纤维素酶液提取葡萄皮渣可溶性膳食纤维的最佳工艺组合为：纤维素酶用量2.0%，提取温度55 ℃，提取时间210 min，料液比1∶20。（2）在最佳工艺条件下，酸法提取8种酿酒葡萄皮渣中可溶性膳食纤维含量占葡萄皮渣干质量的27%～45%；纤维素酶液提取8种酿酒葡萄皮渣中可溶性膳食纤维含量占葡萄皮渣干质量的24%～42%。佳美葡萄所得的SDF含量最高，分别为455.2和421.0 mg/g，其次为霞多丽（438.6 和401.8 mg/g），而西拉最低，分别为277.2 和242.8 mg/g。【结论】HCl与纤维素酶液提取葡萄皮渣中可溶性膳食纤维是可行的，且HCl提取的可溶性膳食纤维的产量普遍高于纤维素酶液，但差异不显著。 |
| 关键词: 葡萄皮渣 可溶性膳食纤维 酸法 酶法 提取工艺 |
| DOI： |
| 分类号: |
| 基金项目:现代农业产业技术体系建设项目（［2007］12号 中国农业部） |
|
| Extraction technology of soluble dietary fiber from grape pomace |
|
|
| Abstract: |
| 【Objective】The aim of this paper was to extract the soluble dietary fiber from pomaces of red grape (Cabernet Sauvignon) from residues of the wine industry with HCl or cellulose enzyme,and compare the contents’differences of eight different grapevine pomaces.【Method】Two methods which used HCl and cellulose enzyme to extract soluble dietary fiber from grape pomaces have been taken to provide basic information on the extraction of soluble dietary fiber.The L9(34) orthogonal test was performed to determine the optimum technique for soluble dietary fiber from the grape pomace adopting HCl or cellulose enzyme.The infected factors were concentration of HCl,concentration of enzyme,extracting temperature,extracting time and ratio of solid to liquid.Comparison of the yields of soluble dietary fiber extracted from eight different grape pomaces were performed by the the optimum technique.【Result】The result of the first trial indicated that the concentration of HCl was marinated in 0.389 mol/L,1∶20 of the ration of material to liquor,75 ℃ for 75 min.The result of the second orthogonal test indicated that the pomaces were marinated in 2.0% of cellulose enzyme,1∶20 of the ratio of material to liquor,55 ℃ for 210 min.The yield of soluble dietary fiber of Gamay Noir which extraced soluble dietary fiber with HCl or enzyme was the highest in eight different grapevine pomaces,which was 455.2 and 421.0 mg/g,and the second Chardonnay’s (438.6,401.8 mg/g）,the least one Syrah(277.2 and 242.8 mg/g).【Conclusion】The suitable technology of soluble dietary fiber extracted from grape pomaces was determined.Comparing the methods which used HCl and cellulose enzyme,the contents of soluble dietary fiber with HCl were higher than thaet of the enzyme’s,and the difference wasn’t significant. |
| Key words: grape pomace soluble dietary fiber extracting method with hydrochloric acid extracting method with enzyme extracting procedure |
