| 摘要: |
| 【目的】从“索邦”百合(Lilium orential“Sorbonne”)中克隆查尔酮合成酶基因启动子相似序列PCHS2，并对其进行表达模式分析。【方法】从“索邦”百合中PCR扩增PCHS2启动子序列，与GUS报告基因融合，构建植物表达载体，通过农杆菌介导法转化模式植物拟南芥（Arabidopsis thaliana var.Columbia）和矮牵牛（Petunia hybrida “Dreams Midnight”），抗性筛选和PCR检测鉴定转基因植株，GUS组织化学检测分析启动子在转基因植株中的表达模式。【结果】转基因拟南芥和矮牵牛的抗性筛选和PCR检测结果显示，成功地获得了转基因阳性植株。GUS活性分析表明，在PCHS2的驱动下，仅能在花药和雌蕊中检测到GUS活性，茎、叶和其他花组织中都没有发现GUS活性的表达。【结论】 PCHS2启动子具有花药专一性。 |
| 关键词: “索邦”百合 查尔酮合成酶基因 花药和雌蕊特异性启动子 矮牵牛 拟南芥 |
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| 基金项目:国家自然科学基金项目“花色形成关键基因特异启动子功能与应用研究”(30871734) |
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| Characterization of the anther and stigma specific promoter from Lily |
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| Abstract: |
| 【Objective】This paper aimed to isolate the CHS promoter like sequence from Lilium orential “Sorbonne” and identify its expression pattern.【Method】 The PCHS2 promoter cloned from Lilium orential “Sorbonne” was used to control the GUS gene expression and transferred to Petunia hybrida “Dreams Midnight” and Arabidopsis thaliana var.Columbia using Agrobacteriummediated method.The transformant plants were assayed by resistance selection and PCR.GUS histochemical localization was used to characterization the expression pattern of PCHS2 promoter in transformant plants.【Result】 Resistance selection and PCR analysis of transgenic plants of Arabidopsis thaliana and Petunia hybrida showed that the transgenic plants were obtained successfully.GUS activity assays indicated that the PCHS2 promoter detected was only expressed in anthers and pistils,whereas no expression was detected in stems,leaves and other flower tissues.【Conclusion】 The PCHS2 promoter determined the anther and pistil specific expression. |
| Key words: Lilium orential “Sorbonne” CHS anther and stigma specific promoter Petunia hybrida Arabidopsis thaliana |
