| 摘要: |
| 【目的】鉴定鸡13号染色体上影响鸡生长性状的数量性状位点（QTL）。【方法】以固始鸡、安卡鸡资源群为基础，在鸡13号染色体已报道的QTL范围内选取4个微卫星标记，采用方差分析方法，对测量的F2代共849个个体的32种生长性状（0,2,4,6,8,10,12周龄体质量，0,4,8,12周龄胫长，4,8,12周龄胫围、胸深、胸宽、胸骨长、胸角、体斜长、骨盆宽等）与4个标记进行相关性分析，对显著影响生长性状的微卫星标记进行不同基因型间各性状最小二乘均值的多重比较；并基于最小二乘区间定位法进行基因组扫描，对生长性状进行QTL初步定位。【结果】在F2群体中检测到的4个微卫星标记平均基因杂合度为0.707，平均多态信息含量为0.653；方差分析显示，各个标记与不同生长性状存在不同程度的相关；定位结果显示，在13号染色体上共检测到6个影响生长性状的QTL，其中3个QTL的影响达极显著水平（P＜0.01）。【结论】将影响4周龄体斜长、胸骨长，8周龄体斜长，6周龄体质量的QTL均定位在13号染色体的52 cM处；而影响0周龄胫长、12周龄胸宽的QTL则分别定位于26和9 cM处。 |
| 关键词: 鸡F2群体 微卫星 生长性状 相关分析 QTL |
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| 基金项目:国家科技支撑计划项目（2008BADB2B07-5）；国家公益性行业（农业）科研专项（nyhyzx07-039）；国家蛋鸡产业技术体系项目；国家农业科技成果转化资金项目（2009D00103）；河南省杰出人才创新基金项目（074200510009） |
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| Identification of QTL on chromosome 13 for growth traits in F2 resource population of chickens |
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| Abstract: |
| 【Objective】The study was to detect quantitative trait loci (QTL) for growth traits on chromosome 13 of chicken.【Mehtod】Four microsatellite markers on chromosome 13 selected from poultry genome were used to study the genetic diversity in 849 F2 individuals based on a chicken resource population from a cross of Gushi chicken representing a slow growing Chinese native breed and Anka (A) broilers representing a fast growing broiler.Thirty two growth traits at different weeks in this study were measured in the F2 population,which included body weight (BW) and body size index containing shank length (SL),shank circle (SC),chest depth (CD),chest width (CW),breast bone length (BBL) and body slanting length (BSL) at different ages.The multi comparison was applied between traits and the genotypes of microsatellite which significantly affected growth traits.Then genome scan was performed to locate the QTL for growth trait by an interval mapping based least squares linear regression.【Result】Average heterozygosity,and average polymorphism information content were 0.707 and 0.653,respectively.Six QTL on growth traits were detected on chromosome 13,three of them were at the 1% genome wide significant level.【Conclusion】The QTL on BSL4,BBL4,BSL8,BW6 were located at the same location 52 cM,and the QTL related to SL0,CW12 were detected at 26,9 cM respectively. |
| Key words: chicken F2 resource population microsatellite growth trait correlation analysis QTL |
