引用本文:
【打印本页】   【下载PDF全文】   查看/发表评论  下载PDF阅读器  关闭
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 1971次   下载 1115 本文二维码信息
码上扫一扫!
分享到: 微信 更多
沙棘林土壤微生物多样性研究
李玉新, 赵 忠, 陈金良
西北农林科技大学 西部环境与生态教育部重点实验室
摘要:
【目的】研究不同林龄沙棘人工林土壤微生物多样性的差异,为沙棘林分的衰败机制研究奠定基础。【方法】采用BIOLOG-ECO微平板法,对不同林龄(8,13,18年生)沙棘人工林表层土(0~10 cm)微生物利用碳源的动力学特征和土壤微生物多样性进行研究,比较分析土壤微生物利用碳源能力、土壤微生物多样性与沙棘人工林林龄的相关性。【结果】18年生沙棘人工林表层土在短期内(培养24~60 h)对单一碳源底物的利用能力高于13和8年生沙棘林表层土,说明13和8年生沙棘人工林表层土中微生物生长的启动速度较慢,生长停滞期较长。随着林龄的延长,土壤微生物群落的Shannon指数(H′)、Simpson指数(D)和Mcintosh指数(U)的变化规律不同,13年生沙棘人工林表层土微生物群落的D、H′值均最大,但U值最小;8和18年生沙棘人工林表层土微生物群落的D、H′、U值差异很小。13年生沙棘人工林表层土微生物利用羧酸类化合物的能力,在培养72 h后相对于8和18年生沙棘人工林均有明显提高;8和18年生沙棘人工林表层土微生物利用醣类化合物的能力,在培养24~144 h时相对于13年生沙棘人工林有明显优势。在其他化合物的利用能力上,8,13和18年生沙棘人工林表层土微生物未表现出明显的差异。只有18年生沙棘人工林表层土在主成分1上可以与13年生沙棘人工林表层土很好地分离,其他林龄沙棘林表层土在主成分1,2,3,4上均未实现分离。【结论】沙棘林的微生物多样性与其林龄存在一定关系。
关键词:  沙棘林  林龄  表层土  微生物功能多样性
DOI:
分类号:
基金项目:国家“十一五”林业科技支撑计划项目(2006BAD03A1207)
Study on soil microbial diversity of seabuckthorn forest
Abstract:
【Objective】The comparative study on soil microbial diversity in seabuckthorn plantations of different ages is to provide theoretical basis for the decay mechanism of seabuckthorn forest.【Method】BIOLOG-ECO micro-plate methodology was taken to study dynamic characteristics of micro organisms using carbon and soil microbial diversity of seabuckthorn plantations in different ages(8,13,18-year)topsoil(0-10 cm),a comparative analysis of the relationship among ability of use carbon,soil microbial diversity and seabuckthorn plantation’s age.【Result】 In the short term(incubation 24-60 h),a single carbon substrate utilization capacity of 18-year topsoil of seabuckthorn plantations is more than that of 13-year and 8-year topsoil,indicating that 13-year,8-year microbial growth of surface soil starts slowly,and microbial stagnation lasts longer;With the extension of the stand age,the Shannon index(H′),Simpson index(D) and Mcintosh index(U) of soil microbial communities shows a different regularity.The D index and the H′ index of 13 year topsoil of seabuckthorn plantations microbial communities are the largest,but the U index is the smallest,the D index,H′ index,U index of 8 year,18-year topsoil of seabuckthorn plantations microbial communities make little difference;Compared to 8-year and 18-year the capable of using carboxylic acids of topsoil micro-organisms,13-year seabuckthorn plantations after 72 h culture has a clear advantage.8,18-year seabuckthorn plantations on topsoil microorganisms in the use of capacity of sugar compounds form 24 h to 144 h has an obvious advantage than 13 year.The capacity of using other compounds on topsoil microorganisms of seabuckthorn plantation in different ages doesn’t show a great difference;Only 18-year on the main component PCA1 of topsoil has a good separation from 13-year,others are not met.【Conclusion】 Difference in microbial diversity has a certain relationship with seabuckthorn’s ages.
Key words:  seabuckthorn forest  stand age  topsoil  microbial functional diversity