| 摘要: |
| 【目的】基于RNA干涉文库，寻找与秀丽线虫缺氧损伤表型相关的剪接调节因子。【方法】利用喂饲RNA干涉(RNA interference,RNAi)文库筛选技术，对秀丽线虫基因组中的351个RNA结合蛋白(RNA binding protein，RBP)基因进行筛选。【结果】经过2次筛选共得到5个与缺氧相关的RBP编码基因，其中剪接因子1个，参与mRNA翻译过程的基因4个。【结论】剪接调节因子可能在mRNA的剪接和翻译水平上参与低氧应答。 |
| 关键词: 喂饲RNAi文库筛选 秀丽线虫 缺氧 RNA结合蛋白 |
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| 基金项目:国家“973”计划项目(2006CB504100)；国家科技重大专项(2009ZX09103-616，2009ZX09301-002，2009ZX09503-002)；国家自然科学基金项目(30900862，30771230，30600365)；中国博士后科学基金项目(200801495，20060400051)；医学神经生物学国家重点实验室开放基金项目(09-02) |
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| Screening of the hypoxia related splicing regulatory factors of C.elegans |
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| Abstract: |
| 【Objective】The study was to find the splicing regulatory factors related to hypoxic injury phenotype.【Method RNA mediated interference (RNAi) library was used to target 351 RNA binding protein (RBP)coding genes of C.elegans.【Result】RNAi knockdown of 5 hypoxia-related RBP genes was obtained,with a splicing factor and 4 genes involved in mRNA translation process.【Conclusion】Splicing regulatory factors may participate in the process of mRNA splicing and translation in hypoxic response. |
| Key words: screening of feeding RNAi library Caenorhabditis elegans hypoxia RNA-binding protein |
