| 摘要: |
| 【目的】研究79份不同产地粗山羊草（Aegliops tauschii）醇溶蛋白的遗传多样性，为粗山羊草在小麦育种中的进一步开发和利用提供理论基础。【方法】利用酸性聚丙烯酰胺凝胶电泳(Acid polyacrylamide gelelectrophoresis，A-PAGE)技术，分析了79份不同产地粗山羊草醇溶蛋白的谱带类型，并进行聚类分析。【结果】从79份粗山羊草中共检测出70条相对迁移率不同的醇溶蛋白谱带，各谱带出现的频率为1.27%～77.22%，每份材料谱带数为7～19条，平均为12.58条，遗传多样性指数为0.055 3～0.367 6。根据迁移率大小，在ω、γ、β和α 4个区分别存在20，13，21和16种谱带类型。UPGMA聚类分析表明，79份粗山羊草在遗传相似系数(GS)为0.78时，聚为7个主要类群，大部分相同产地的粗山羊草聚为一类，但也有少部分产地相同的材料不全聚为一类。部分来源地不同的材料醇溶谱带完全相同。【结论】79份粗山羊草醇溶蛋白遗传多样性丰富，其醇溶蛋白的谱带类型与材料产地具有一定的相关性，也有少部分表现出一定的差异性，其中醇溶谱带完全相同的材料可能含有相同醇溶蛋白编码基因，其亲缘关系可能较近。 |
| 关键词: 粗山羊草 醇溶蛋白 A PAGE UPGMA法 遗传多样性 |
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| 基金项目:陕西省作物种质资源收集与创新利用平台建设（农业部948项目）；西北农林科技大学唐仲英育种基金项目 |
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| Genetic diversity of gliadin in Aegliops tauschii |
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| Abstract: |
| 【Objective】Gliadin genetic diversity of 79 different places Aegliops tauschiiwas studied to provide theoretical basis for further study and utilization of A.tauschii.【Method】The standard gliadin fingerprints and database of 79A.tauschiiaccessions were analyzed by A PAGE,and cluster analysis was performed.【Result】70 bands of different relative mobility in 79 A.tauschiiwere detected,and each band frequency range of variation was 1.27%-77.22%,the number of bands for each material 7 to 19,with an average of 12.58,genetic diversity index range of 0.055 3-0.367 6.According to the size of mobility,20,13,21 and 16 types of bands were found in the ω,γ,β,and α 4 zones.UPGMA cluster analysis showed that 79 copies of A.tauschiigenetic similarity coefficient (GS) was 0.78,and clustered into seven major taxa,most of the same origin of A.tauschiiclustered into one class,but a small part of the same origin didn’t while some materials of different origins shared identical bands.【Conclusion】The alcohol-soluble protein bands and material origin had a certain relevance,but some small differences also showed,materials of identical gliadin bands may contain the same genencoding gliadin, their phylogenetic relationship may be close. |
| Key words: Aegliops tauschii gliadin A-PAGE UPGMA method genetic diversity |
