摘要: |
【目的】探讨外源NO对NaCl胁迫下红三叶(Trifolium pratense)幼苗生长和光合的缓解效应。【方法】用100 mmol/L NaCl胁迫红三叶幼苗,分别采用50,100和150 μmol/L硝普钠 (Sodium nitro prusside,SNP)处理,研究红三叶幼苗生长及净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)和胞间CO2浓度(Ci)等光合指标的变化。【结果】100 μmol/L SNP处理能显著(P<0.05)缓解100 mmol/L NaCl胁迫对红三叶幼苗生长的抑制作用,尤其对地下部生长抑制的缓解效果更为明显;可显著提高NaCl胁迫下幼苗叶片的净光合速率(Pn)、蒸腾速率(Tr)和气孔导度(Gs),降低胞间CO2浓度(Ci)。【结论】外源NO可缓解NaCl胁迫对红三叶幼苗生长及光合作用的抑制作用,且以100 μmol/L SNP的缓解效果较好。 |
关键词: 红三叶 NaCl胁迫 一氧化氮(NO) 幼苗生长 光合特性 |
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基金项目:陕西省自然科学基金项目(2009JM3015);陕西省科技攻关项目(2009K01-19) |
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Effect of exogenous nitric oxide on the growth and photosynthetic characteristics of Trifolium pratense seedings under NaCl stress |
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Abstract: |
【Objective】The objective of this study was to investigate the effects of exogenous nitric oxide on seedlings growth and photosynthetic characteristics of red clover (Trifolium pratense) seedlings under NaCl stress.【Method】Using 50,100 and 150 μmol/L SNP (sodium nitroprusside),an exogenous nitric oxide donor as NO supply body was used to study the red clover seedling growth,net photosynthetic rate,transpiration rate and other photosynthetic indexes under 100 mmol/L of NaCl stress.【Result】100 μmol/L SNP treatment could significantly alleviate the inhibitory effect of seedlings growth under the 100 mmol/L of NaCl stress (P<0.05),and alleviate the effects of underground growth significantly better than on the ground growth;it could significantly increase net photosynthetic rate (Pn),stomatal conductance (Gs) and transpiration rate(Tr),lower intercellular CO2 concentration (Ci) in leaves of red clover.【Conclusion】SNP can alleviate the inhibition effects under NaCl stress of the seedlings growth and photosynthesis of T.pratense significantly,and the best SNP treatment to relieve the stress of NaCl on the growth and photosynthetic characteristics of T.pratense’s seedings is the concentration of 100 μmol/L. |
Key words: red clover(Trifolium pratense) NaCl stress nitric oxide (NO) seeding growth photosynthetic characteristic |