| 摘要: |
| 【目的】探讨微囊藻毒素(MC)对大型溞摄食行为的影响。【方法】采用急性毒性试验确定MC对大型溞的半数致死浓度(LC50)，以此为依据，设计含0.00(正常组),24.49,36.74,55.11,82.67和124.00 μg/L MC的培养液培养大型溞，并以小球藻饲喂，检测处理前后及恢复条件(在不含MC的培养液中培养)下，母代和子代大型溞滤水率和摄食率的变化。【结果】MC处理可显著降低大型溞母代和子代的滤水率及摄食率，且降低程度与MC质量浓度呈正相关，其中对子代摄食强度的抑制大于母代；恢复条件下大型溞子代的摄食强度有所回升，但与正常组相比仍明显偏低。MC对大型溞母代、子代第1胎、子代第3胎的滤水率和摄食率达到半数抑制时的质量浓度分别为54.75和75.61 μg/L，44.42和52.78 μg/L，39.47和47.01 μg/L。【结论】MC可严重抑制大型溞的摄食行为。 |
| 关键词: 微囊藻毒素 大型溞 滤水率 摄食率 |
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| 基金项目:河南省动物学重点学科基金项目；河南师范大学青年科学基金项目(2009qk18) |
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| Effects of microcystin on the feeding behavior of Daphnia magna |
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| Abstract: |
| 【Objective】This article investigated the effects of microcystin (MC) on feeding behavior of Daphnia magna.【Method】Initially an acute toxicity test was conducted to determine the median lethal concentration (LC50) values of MC to D.magna(age＜24 h).Then the organisms were exposed to different concentrations of MC (0.00（control）,24.49,36.74,55.11,82.67 and 124.00 μg/L) with Chlorella vulgaris as the food to examine the filtration and ingestion rates of F0 and F1 generations of D.magna.A contrast experiment of transferring the FF1 generation to recovery medium (MC-free) was also carried out.【Result】The results showed that MC could significantly inhibit the filtration and ingestion rates of D.magna,following higher MC concentrations with stronger inhibition.And the inhibition on FF1 generation was more significant than F0 generation.Additionally,the feeding intensity of F1 generation cultured in recovery medium decreased significantly than that in the control.The median effective concentrations (EC50) of MC on filtration and ingestion rates of F0,F1 1st and 3rd generations of D.magna were 54.75 and 75.61 μg/L,44.42 and 52.78 μg/L,39.47 and 47.01 μg/L,respectively.【Conclusion】These results suggest that MC can strongly influence the feeding behavior of D.magna. |
| Key words: microcystin Daphnia magna filtration rate ingestion rate |
