| 摘要: |
| 【目的】验证从抗逆植物柽柳中克隆得到的rd22基因的耐盐能力。【方法】采用根癌农杆菌介导法，对烟草进行rd22基因的遗传转化，将分子检测为阳性的6个转基因烟草株系和非转基因对照烟草的组培苗进行盐胁迫（NaCl浓度分别设为0（CK），110，220和330 mmol/L）试验，通过观察相对电导率、SOD活性和相对生长量的变化，研究rd22基因的耐盐能力。【结果】生物信息学分析表明，来自柽柳的rd22基因属于BURP蛋白家族。分子检测证明，该rd22基因已整合到烟草基因组中，并在mRNA水平上有不同程度的表达。相对电导率和SOD活性分析表明，随着盐胁迫浓度的增加，各转基因烟草株系的相对电导率均呈上升趋势，且均小于对照烟草，在盐胁迫浓度达到330 mmol/L时，非转基因对照烟草的相对电导率高达16.49%，而各转基因烟草的相对电导率最大为14.91%。各转基因烟草株系的SOD活性也随着盐胁迫浓度的增加均呈上升趋势，且均大于对照烟草，在盐胁迫浓度达到330 mmol/L时，转基因烟草SOD活性是对照烟草的1.10～1.39倍。相对生长量分析表明，盐胁迫浓度达到110，220，330 mmol/L时，各转基因烟草株系相对生长量均高于对照株系，非转基因烟草的相对生长量分别为59.92%,36.99%和12.72%，转基因烟草的相对生长量平均为67.00%，44.05%和17.47%。【结论】rd22基因的导入，提高了转基因烟草的耐盐性。 |
| 关键词: 柽柳 rd22基因 生物信息学 遗传转化 耐盐性 |
| DOI： |
| 分类号: |
| 基金项目:科技部转基因生物新品种培育重大专项(2009ZX08009-098B)；中央高校基本科研业务费专项资金项目（DL09BA22） |
|
| Sequence analysis and salt tolerance of rd22 gene from Tamarix androssowii |
|
|
| Abstract: |
| 【Objective】The study was done to observe tolerant extent of transgenic tobacco with rd22gene from Tamarix androssowii towards salt stress.【Method】Using Agrobacterium mediated transformation systems,rd22 gene was integrated into tobacco genome,and was detected with RT-PCR,then six transgenic tobaccos and control plants were treated with salt stress（0(CK),110,220,330 mmol/L NaCl） to measure the relative growth and relative electrical conductivity and SOD-like activity in order to investigate the salt tolerance of rd22 gene.【Result】The rd22 gene is a member of BURP family according to bioinformaties analysis.By realtime quantitative RT-PCR,rd22 gene was integrated into tohacoo genome and expressed diversely in mRNA level.The results of the electrical conductivity and SOD-like activity showed:the relative electrical conductivity of the transformed tobacco lines was lower than that of the control line,and the conductivity increased along with the increase of the salt stress.Under 330 mmol/L NaCl,the highest of conductivity of the transgenic tobacco lines was 14.91% and the control line was 16.49%;The SOD-like activity of the transformed tobacco lines was higher than that of the control line,and the contents increased along with the tobacco lines 1.10-1.39 times more than the control line.The results of the relative growth showed:under 110,220,330 mmol/L NaCl,the relative growth of transgenic tobacco lines was higher than that of the control line,and the relative growth of control line was respectively 59.92%,36.99%,12.72%,and the transgenic tobacco lines respectively 67.00%,44.05%,17.47%.【Conclusion】The results show that rd22 gene transferred into the tobacco enhances the resistance of the tobacco towards salt stress. |
| Key words: Tamarix sp. rd22 gene bioinformatics genetic transformation salt tolerance |
