| 摘要: |
| 【目的】 研究人PDX-1基因对胰岛发育相关基因和胰岛素基因（Insulin，INS)表达的影响，探讨PDX-1基因诱导非β细胞为胰岛素分泌细胞的可能性。【方法】 采用PCR扩增技术，从人的胰腺cDNA中扩增PDX-1基因和INS基因的阅读框架，分别构建了质粒pAAV-PDX-1和pAAV-INS；将pAAV-PDX-1转染人胚肾细胞 (293T) 48 h后，RT-PCR检测293T细胞中胰岛发育相关基因的表达；再将pAAV-PDX-1和 pAAV-INS共转染293T细胞48 h后，RT-PCR检测293T细胞中INS的表达。【结果】 转染pAAV-PDX-1的293T细胞中，检测到胰岛发育相关基因PDX-1、Ngn3、PC2、BETA2的表达；PDX-1没有直接增强INS基因表达的作用。【结论】 在293T细胞中，PDX-1基因对胰岛发育相关基因的表达有重要影响，PDX-1在诱导非β细胞为胰岛素分泌细胞的过程中可能有着重要的作用。 |
| 关键词: pAAV-PDX-1；pAAV-INS；293T细胞 胰岛发育相关基因；表达 |
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| 基金项目:国家“973”项目（2007CB947900,2007CB948103）；国家“863”项目(2006AA02A102)；国家自然科学基金项目（30800650） |
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| Effect of PDX-1 on the expression of pancreas islet development related genes |
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| Abstract: |
| 【Objective】 The research was conducted to study the function of the PDX-1 gene and the effect of PDX-1 gene on regulation of INS gene expression in non β-cells.【Method】 The plasmid pAAV-PDX-1 was transfected into the 293T cells and the expression of the pancreas islet development related genes was analyzed by RT-PCR.The plasmid pAAV-PDX-1 and pAAV-INS were transfected into the 293T cells and the expression of the INS gene was analyzed by RT-PCR.【Result】 Some insulin secreting cell related genes (PDX-1,Ngn3,PC2,BETA2) were expressed in the 293T cells transfected with pAAV-PDX-1.There was no detectable change in the expression of the INS gene in the 293T cells co-transfected with pAAV-PDX-1 and pAAV-INS compared to the cells transfected with pAAV-INS or the cells co-transfected with pAAV-PDX-1 and pAAV-INS.【Conclusion】 The PDX-1 gene affects the expression of the Insulin secreting cell related genes in 293T cells and plays a potential role in the induction of non β-cells into the insulin secreting cells. |
| Key words: pAAV-PDX-1 pAAV-INS 293T cell the pancreas islet development related gene expression |
