| 摘要: |
| 【目的】 探讨猪附红细胞体病对仔猪非特异性免疫功能的影响，并筛选有效的免疫调节剂作为辅助治疗药物。【方法】 选取20头40日龄个体质量相近、无附红细胞体感染的健康仔猪，随机分为5组，1~3组为试验组，4组为阳性对照组，5组为阴性对照组。1~4组人工接种附红细胞体菌血5 mL/头，5组同时注射等量生理盐水。随后1~3试验组分别给予防风多糖溶液0.1 mL/kg、黄芪多糖注射液0.1 mL/kg和左旋咪唑注射液0.05 mL/kg，阴性和阳性对照组给予生理盐水各0.1 mL/kg，连用7 d,分别于第0，4，7，14天自猪前腔静脉采血，测定红细胞感染率、血清溶菌酶活力、淋巴细胞总数、血清总蛋白含量、血清球蛋白含量、血清免疫抑制酸性蛋白（IAP）含量。【结果】 阳性对照组、防风多糖组、左旋咪唑组和黄芪多糖组的红细胞感染率分别为62.75%，52.25%，51.25%和40.00%。仔猪感染附红细胞体后，阳性对照组血清总蛋白含量、球蛋白含量显著降低，IAP含量显著升高（P＜0.05），淋巴细胞总数略有下降，血清溶菌酶活力无显著变化（P＜0.05）。黄芪多糖、左旋咪唑能显著提高感染仔猪的血清总蛋白含量和溶菌酶活力（P＜0.05），3种免疫调节剂均能显著提高感染仔猪的血清球蛋白含量（P＜0.05），降低IAP含量（P＜0.05）。【结论】 3种免疫调节剂均能提高感染仔猪的非特异性免疫，其中以黄芪多糖的效果最好。 |
| 关键词: 附红细胞体病 仔猪 非特异性免疫 免疫调节剂 |
| DOI： |
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| 基金项目:山东省科技攻关项目（GG200730009011） |
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| Selection of effective immunomodulators for eperythrozoonosis |
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| Abstract: |
| 【Objective】 The experiment was conducted to study effects of eperythrozoonosis on nonspecific immunity of piglets and select effective immunomodulators.【Method】 A total of 20 healthy piglets with similar body weight,aged 40 days,were assigned to 5 treatment groups randomly.The first 3 groups were experimental groups,the 4th positive control and the 5th negative control.Piglets of the first 4 groups were inoculated with porcine blood containing Eperythrozoon suis,and piglets of the 5th group were simultaneously treated with saline.Then,piglets of experimental groups were intramuscularly injected with Polysaccharide of Radix Sileris (PRS),Astragalus Polysacharin (APS) and Levamisole (LMS) respectively,and piglets of controls were simultaneously treated with saline for 7 days.Blood was sampled from precaval vein on the 0th,4th,7th and 14th day after inoculation.Lisozyma activity,total lymphocyte count,total protein level, globulin level and immunosuppressive acid protein (IAP) level were tested.【Result】 Positive,PRS,LMS,APS red blood cell infection was 62.75%,52.25%,51.25%,40.00%.The results showed that eperythrozoonosis infection significantly decreased total protein level and globulin level,significantly enhanced IAP level,and gently decreased total lymphocyte count of piglets of positive.But there was no significant change of lisozyma activity.APS and PRS could significantly increase total protein level and lisozyma activity,and all of the three immunomodulators could significantly increase globulin level and decrease IAP level of infectious piglets.【Conclusion】 All the three immunomodulators could increase nonspecific immunity of piglets,and APS was the best. |
| Key words: eperythrozoonosis piglet nonspecific immunity immunomodulator |
