| 摘要: |
| 【目的】分析心脏脂肪酸结合蛋白（H-FABP）和脂肪细胞型脂肪酸结合蛋白（A-FABP）基因mRNA在不同日龄清远麻鸡不同组织中的差异表达量，为进一步阐明H-FABP、A-FABP在鸡IMF含量和腹脂沉积中的作用机制奠定基础。【方法】 依据鸡H-FABP和A-FABP基因及参照基因GAPDH序列设计引物，以鸡心肌、胸肌、腿肌、肝脏和腹脂mRNA逆转录合成的cDNA为模版，应用SYBR GreenⅠ实时荧光定量PCR，检测56和120日龄清远麻鸡A-FABP、H-FABP基因mRNA的差异表达量。【结果】 H-FABP、A-FABP和GAPDH的基因融解曲线均为单峰，无杂峰及二聚体，定量准确。120 d时清远麻鸡的胸肌和腿肌的肌内脂肪（IMF）含量显著高于56 d；H-FABP基因mRNA 120 d时在心肌、胸肌和腿肌的差异表达量显著高于56 d，且同一日龄时在心肌的差异表达量显著高于胸肌和腿肌(P＜0.05)。120 d时清远麻鸡A-FABP基因mRNA在腹脂和肝脏的差异表达量显著高于56 d；同一日龄时，A-FABP基因mRNA在腹脂的差异表达量显著高于肝脏，A-FABP基因在心肌、胸肌和腿肌中不表达。相关分析表明，H-FABP基因mRNA差异表达量与胸肌、腿肌IMF含量分别呈显著和极显著正相关，A-FABP基因mRNA差异表达量与腹脂率呈极显著负相关。【结论】 H-FABP基因主要在鸡肌肉组织的肌内脂肪中表达，而且在心肌中高度表达，与IMF含量呈显著或极显著的正相关，其是IMF含量的下调基因；A-FABP基因主要在腹脂与肝脏中表达，且在腹脂中高度表达，与腹脂率呈极显著负相关，是脂肪细胞的上调基因，且为正调控。 |
| 关键词: 清远麻鸡 荧光定量逆转录PCR A-FABP基因 H-FABP基因 差异表达 |
| DOI： |
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| 基金项目:国家科技支撑计划项目（2006BDA01A09，2008BADC1B01，2008BADC1B03）;国家“863”计划项目（2008AA101009-7） |
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| Study on the relative H-FABP and A-FABP mRNA gene expression in Qingyuan partridge |
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| Abstract: |
| 【Objective】 Heart fatty acid binding protein(H-FABP) and adipocyte fatty acid binding protein and （A-FABP)gene expression of different tissues were analysed in Qingyuan patridge chicken at different ages.The study will establish a basis for clarifying H-FABP and A-FABP gene expression mechanism in IMF and abdomen fat deposition.【Method】 The primers were designed according to the sequences of H-FABP,A-FABP and GAPDH gene.The c-DNA fragment amplified from leg muscle,breast muscle,cardiac muscle,liver and abdominal fat was used as a standard template；H-FABP and A-FABP gene expression was detected using SYBR Green I real time fluorescent quantification PCR.The relative expression in H-FABP，A-FABP gene was detected using real time quantitative RT-PCR in Qingyuan patridge chicken.【Result】 Melting curve analysis showed a single peak of the genes.IMF content in breast muscle and leg muscle of Qingyuan partridge on 120 d was higher than that of 56 d.However,the relative H-FABP mRNA level in these tissues was significantly lower than that of Qingyuan partridge on 56 d.The relative H-FABP mRNA level in Cardiac muscle was significantly higher than that of breast muscle and leg muscle.The relative A FABP mRNA levels of abdomen fat and liver were significantly higher than that of the other tissues(P＜0.05).The relative A-FABP mRNA level in abdomen fat was higher than that in liver.H-FABP mRNA expression level was significantly postive to IMF contents.A-FABP mRNA expression level was significantly negative to abdomen fat and liver.【Conclusion】 H-FABP gene mRNA was expressed in IMF of different muscle tissues.Moreover，the relative H-FABP mRNA level was much higher in Cardiac muscle.H-FABP mRNA expression level was significantly positive to IMF.Furthermore,it had negative effect on IMF content.A-FABP gene mRNA was expressed in abdomen fat and liver.Moreover，the relative A-FABP mRNA levels in abdominal fat was higher.A-FABP mRNA expression level was significantly negative to abdominal fat.A-FABP mRNA expression level had a significantly positive effect on abdominal fat rate. |
| Key words: Qingyuan partridge chicken FQ-RT PCR A-FABP gene H-FABP gene gene expression |
