| 摘要: |
| 【目的】 研究环塔里木盆地果、棉间作棉花品种的遗传多样性，为该地区间作棉花品种的培育和选配提供理论依据。【方法】 应用RAPD标记技术，对环塔里木盆地23个果、棉间作棉花品种的遗传多样性及其相似性进行分析，并对其分子聚类结果进行探讨。【结果】 经筛选发现，在70个随机引物中，有27个引物能在23个间作棉花品种间扩增出较好的多态性片段，共扩增出136条多态性条带，占总条带数的67.3%。环塔里木盆地23个间作棉花品种之间的平均成对相似系数为0.665，成对相似系数大于0.7的品种对有66个，占总数的26.09%，而成对相似系数小于0.5的品种对仅占总数的6.72%。23个棉花品种可聚为2个大类5个亚类。【结论】 环塔里木盆地果、棉间作棉花品种遗传相似性较高，遗传基础比较狭窄。 |
| 关键词: 果棉间作 棉花品种 RAPD标记 遗传多样性 分子聚类分析 |
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| 基金项目:“十一五”国家科技支撑计划项目（2007BAD36B03-3） |
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| Analysis of genetic diversity in cotton varieties of intercropping of apricot and cotton around Tarim Basin |
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| Abstract: |
| 【Objective】 This study was to illustrate genetic diversity of main cotton varieties in intercropping of fruit and crop，which provided a theoretical basis for breeding and selection of cotton varieties.【Method】 The randomly amplified polymorphic DNA procedure (RAPD) was used to assess the genetic diversity of 23 cotton varieties of the intercropping in the Tarim Basin in this study,and the software of NTSYS pc version 2.10 was used to calculate similarity coefficients and construct a dendrogram.【Result】 A total of 70 arbitrary primers were screened using RAPD markers with the 23 cotton species and finally 27 primers which could produce steady polymorphism were obtained.136 polymorphic bands were amplified and the polymorphic rate was 67.3%.Mean similarity among 23 cotton varieties of the intercropping was 0.665,in which more than 0.7 accounted for 26.09% and less than 0.5 was only 6.72%.Cluster analysis revealed that these 23 cotton species could be divided into 2 classes and 5 subclasses,which clarified the genetic diversity of these species.【Conclusion】 There was a low level of genetic diversity among these cotton varieties of the intercropping in the Tarim Basin. |
| Key words: intercropping of fruit and crop cotton variety RAPD analysis genetic diversity cluster analysis |
