| 摘要: |
| 【目的】 检测从普通小麦（Triticum aestivum L.）与奥地利黑麦杂交后代选育出的、形态学稳定的抗条锈病衍生系NR1121的黑麦遗传物质。【方法】 在室内条锈病抗性鉴定的基础上，采用细胞学方法、基因组原位杂交、SCAR（Sequence characterized amplified region）标记以及 SSR（Simple sequence repeat）标记、A-PAGE等方法，对普通小麦-奥地利黑麦衍生系NR1121进行分子细胞遗传学鉴定。【结果】 NR1121和奥地利黑麦对条中32号生理小种（CY32）免疫，陕麦611和携带Yr9基因的洛夫林10、洛夫林13、秦麦9号、丰抗8号、陕229和偃师9号均高感，表明NR1121携带的抗条锈病基因来源于奥地利黑麦。NR1121细胞学稳定，根尖细胞染色体数2n=42，花粉母细胞减数分裂期2n=21Ⅱ；以奥地利黑麦总基因组DNA为探针的原位杂交结果显示，NR1121 含有 2 条奥地利黑麦染色体。SCAR和SSR标记(Xgwm 131-1B)检测结果表明，NR1121携带黑麦1R染色体的遗传物质。A-PAGE结果显示，NR1121 在ω区的Gli-B1位点具有黑麦特征带，说明NR1121含有黑麦1R染色体短臂上的遗传物质。【结论】 NR1121为农艺性状优良的小麦 黑麦1R异代换系，对CY32免疫，其抗病基因来源于奥地利黑麦，可能是一个不同于Yr9基因的新抗条锈病基因。该种质可作为条锈病抗源用于小麦抗病育种。 |
| 关键词: 奥地利黑麦 基因组原位杂交 SCAR标记 SSR标记 异代换系 |
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| 基金项目:国家“973”重大基础研究规划项目（2006CB708208）；西北农林科技大学人才基金项目 |
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| Identifications of wheat-Secale cereale derivative NR1121 resistant to stripe rust |
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| Abstract: |
| 【Objective】 The aim of this research was to identify the genetic chromatin transferred to NR1121 from S.cereal.【Method】 The resistance to stripe rust pathogens of NR1121 was identificated.Chromosome analysis,genomic in situ hybridization (GISH),A-PAGE,SSR and SCAR were used to detect S.cereal chromatin in NR1121.【Result】 NR1121 and Austrian rye were immune to isolate CY32 of Puccinia striiformis f.sp.tritici.Shaanmai 611 and six cultivars (Lovrin 10，Lovrin 13，Qingmai No.9，Fengkang No.8，Shaan229 and Yanshi No.9) with Yr9 were susceptible to CY32.The results indicated stripe rust resistanance gene in NR1121 was derived from Austrian rye.NR1121 was stable in cytology with chromosome numbers 2n=42=21Ⅱ.GISH analysis with Austrian rye genomic DNA as a probe showed that the two chromosomes of NR1121 were transferred from S.cereal.SCAR and SSR marker (Xgwm131-1B) detection indicated that NR1121 possessed chromatin of Austrian rye 1R.A-PAGE indicated that Gli-B1 in ω region of NR1121 had the specific band of Austrian rye.The result showed NR1121 had chromatin of Austrian rye 1RS.【Conclusion】 NR1121 was a wheat-S.cereale 1R disomic subsititution line and immune to CY32.The resistant gene in NR1121 was derived from Austrian rye and probably a new gene different from Yr9.NR1121 could be used as resistant resource of stripe rust for wheat breeding |
| Key words: Austria rye(Secale cereale) GISH SCAR SSR alien disomic subsititution line |
