| 摘要: |
| 【目的】 研究N,N-二甲基甲酰胺（N,N-Dimethylformamide, DMF）与甘油配制的混合保护剂对猪冷冻精子的保护效果。【方法】 以DMF和甘油配制成混合冷冻保护剂对猪精液进行冷冻，试验共设对照组（30 mL/L甘油）、A组（10 mL/L DMF+20 mL/L甘油）、B组（20 mL/L DMF+10 mL/L甘油）、C组（30 mL/L DMF）和D组（50 mL/L DMF） 5个处理组，以活动精子百分率、精子活率、精子直线运动速率、精子顶体完整率以及精子质膜完整率为检测指标，对猪精子冷冻效果进行评价。【结果】 20 mL/L DMF+10 mL/L甘油混合冷冻保护剂对猪精子有较好的冷冻保护作用，与其他处理组相比，除精子直线运动速率和质膜完整率与10 mL/L DMF +20 mL/L甘油组无显著差异外，其他各项指标均优于其他组及甘油对照组。30 mL/L DMF对猪精子的毒性强于同浓度甘油，但20 mL/L DMF+10 mL/L甘油混合冷冻保护剂的毒性与甘油对照组无显著差异（P>0.05）。【结论】 20 mL/L DMF+10 mL/L甘油混合冷冻保护剂比单一使用甘油或DMF能起到更好的冷冻保护作用。 |
| 关键词: 猪精液 冷冻保存 N,N-二甲基甲酰胺 精子品质 |
| DOI： |
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| 基金项目:国家“863”计划项目（2008AA101004） |
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| Effect of N,N-Dimethylformamide on cryopreservation of boar semen |
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ZHANG Yue jina ZHANG Xiao yanb PANG Yong qiangb Li Xueb
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| Abstract: |
| 【Objective】 The effect of mixed cryoprotectants composed of different proportions of glycerol and N,N-Dimethylformamide (DMF) on freezing boar sperm was studied.【Method】 The mixing cryoprotectant was composed of DMF and glycerol,and experiments were executed among five treatments: the control (30 mL/L glycerol), group A (10 mL/L DMF +20 mL/L glycerol), group B (20 mL/L DMF +10 mL/L glycerol),group C (30 mL/L DMF) and group D (50 mL/L DMF).In the present study,the effects of mixing cryoprotectant on frozen thawed sperm were evaluated by five sperm parameters including the percentage of total motile sperm,motility,velocity of straight line motion,plasma membrane integrity and acrosome integrity.【Result】 20 mL/L DMF +10 mL/L glycerol could protect boar sperm against cryodamage during cryopreservation. No difference on velocity of straight line motion and membrane integrity of frozen thawed sperm was found between group B and group A (P>0.05),and the parameters of frozen thawed sperm of the group B were higher than those of other groups (P<0.05);The toxicity of DMF was stronger than that of glycerol at the same level of concentration (30 mL/L),and no difference existed between group B and the control in the present study (P<0.05).【Conclusion】 20 mL/L DMF +10 mL/L glycerol could provide better protective action for frozen boar sperm than that of DMF or glycerol alone. |
| Key words: boar semen cryopreservation N,N-Dimethylformamide sperm quality |
