| 摘要: |
| 【目的】 分析获能与冻融猪精子细胞肌动蛋白表达的差异。【方法】 提取获能和冻融的猪精子蛋白，经过SDS-PAGE分离后，进行Western-blot免疫印迹分析，检测获能与冻融猪精子间细胞肌动蛋白的区别。【结果】 新鲜和冻融猪精子细胞肌动蛋白单体的分子质量均约为43 ku，但获能猪精子细胞肌动蛋白单体的分子质量约为43和86 ku。【结论】 冻融的猪精子细胞肌动蛋白单体分子质量约为43 ku，但数量有所下降；获能后的猪精子出现了一种新型肌动蛋白亚单位，其分子质量约为86 ku。 |
| 关键词: 猪精子 获能 冻融 肌动蛋白 |
| DOI： |
| 分类号: |
| 基金项目:“211”工程重点学科项目；延边大学研究生科研项目 |
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| Difference between capacitation and frozen-thawed cell actin expression in boar sperm |
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SONG Zhao peng1 2 JI Xin wei3 WANG Sheng lei4 ZHANG Yong gang1 GONG Chang rong1
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| Abstract: |
| 【Objective】 The study was done in order to analyze the difference between capacitation and frozen-thawed boar sperm cells actin expression.【Method】 After SDS-PAGE separation of the capacitation and frozen-thawed boar sperm,western blotting analysis was carried out to detect the distinction between capacitation and frozen-thawed boar sperm cell actin.【Result】 Monomer molecular weight of fresh and frozen-thawed boar sperm cells actin was about 43 ku,but capacitation boar sperm cells actin was about 43 ku and 86 ku.【Conclusion】 Monomer molecular weight of frozen-thawed boar sperm cells actin was still 43 ku,but there was a decrease in number;a new actin subunit appeared in capacitation boar sperm,and the molecular weight was 86 ku . |
| Key words: boar sperm capacitation frozen-thaw actin |
