| 摘要: |
| 【目的】 研制磺胺间甲氧嘧啶(Sulfamonomethoxine，SMM)残留快速检测阻断ELISA试剂盒（SMM-Kit），为有效治理磺胺间甲氧嘧啶的滥用，保障动物源性食品安全提供技术支撑。【方法】 在成功研制SMM单克隆抗体（Monoclonal antibody,mAb）的基础上，应用阻断ELISA试验原理研制SMM-Kit，并对其特性进行了系统测定。【结果】 SMM-Kit标准曲线呈典型的S型，相关系数R2＝0.992 8，符合4参数logit曲线拟合，线性检测范围为1.33～389.9 μg/L，灵敏度为3.57 μg/L，半数抑制浓度（IC50）为17.07 μg/L。牛奶样和猪尿样的平均添加回收率分别为91.5%和92.0%，平均批内和批间变异系数均低于15%；基质对SMM-Kit检测结果的影响不大；SMM-Kit与其他磺胺类药物的交叉反应性<1.7%，表明其与其他药物几乎无交叉反应性；试剂盒在4 ℃可保存6个月。【结论】 成功地研制出了SMM-Kit，在牛奶和猪尿液样品中的初步应用证明，该试剂盒灵敏度、准确度高，特异性强，可低温保存6个月。 |
| 关键词: 磺胺间甲氧嘧啶 单克隆抗体 阻断ELISA 快速检测试剂盒 |
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| 基金项目:国家“十一五”科技支撑计划项目（2006BAK02A21） |
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| Develepment and trait identification of rapid test blocking ELISA Kit for sulfamonomethoxine residues |
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| Abstract: |
| 【Objective】 A blocking ELISA test Kit for detection of Sulfamonomethoxine（SMM）was developed to offer technological support for effective governance of the abuse of sulfamonomethoxine and protection of animal derived food safety.【Method】 Based on a high sensitivity monoclonal antibody of SMM,a rapid test ELISA Kit was established and identified by blocking ELISA.【Result】 The calibration curve of the SMM-Kit with standard SMM inhibitor was typical sigmoid curve fitted to the four parameter logistic equation with the linear detection of 1.33-389.9 μg/L（R2＝0.992 8），the sensitivity of 3.57 μg/L，the IC50 of 17.07 μg/L.The recoveries of SMM spiked in milk were 91.5%，in pig urine 92.0%.The precision and accuracy of the assay as determined by inter-assay and intra-assay coefficient variation was both below 15%. The SMM-Kit generally had 1.7% cross reactivity towards sulfamerazine and had little or no cross reactivity towards other compounds.The dilution solution of SMM had no effect on results of SMM-Kit. The validity of SMM-Kit in 4 ℃ was above six months.【Conclusion】 A blocking SMM-Kit was established and identified.The successful preliminary application in detection for SMM residues of milk and Urine showed that the SMM-Kit had a high sensitivity,accuracy,specificity and the validity at low temperature was above six months. |
| Key words: sulfamonomethoxine monoclonal antibody blocking ELISA rapid test Kit |
