| 摘要: |
| 【目的】 探讨长春碱聚氰基丙烯酸正丁酯纳米粒（VLB-PBCA-NP）对正常细胞的毒性及其急性毒性。【方法】 采用MTT法并结合形态学观察，比较了VLB-PBCA-NP和VLB原料药对原代大鼠星形胶质细胞和小鼠成纤维细胞L929的毒性；用相同剂量的VLB-PBCA-NP和VLB原料药溶液对小鼠进行尾静脉注射，每日观察小鼠死亡情况，并计算半数致死量（LD50）。【结果】 在质量浓度为0.5~50 ng/mL时，VLB-PBCA-NP和VLB原料药对星形胶质细胞增殖抑制率的影响无显著性差异，当质量浓度达到500 和5 000 ng/mL时，VLB-PBCA-NP对大鼠星形胶质细胞的毒性显著低于VLB原料药（P<0.05）。5 000 ng/mL的VLB-PBCA-NP对小鼠成纤维细胞L929的毒性为3级，极显著降低了VLB原料药的毒性（P<0.01）。VLB-PBCA-NP和VLB原料药的LD50分别为26.38 和22.7 mg/kg，前者较后者提高了16.21%。【结论】 VLB-PBCA-NP对小鼠的急性毒性和对非肿瘤细胞的毒性均显著低于VLB原料药。 |
| 关键词: 长春碱 纳米粒 L929细胞 星形胶质细胞 半数致死量 |
| DOI： |
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| 基金项目:国家科技支撑计划项目（2006BAD04A11） |
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| Cytotoxicity and acute toxicity study on vinblastine (VLB) nanoparticles |
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| Abstract: |
| 【Objective】 The aim of this study was to investigate toxicity of VLB poly(buty1 cyanoacrylate) nanoparticles ( PBCA-NP).【Method】 Difference between VLB-PBCA-NP and crude VLB on the growth of L929 and rat astrocyte was measured by MTT assay and morphology method.In addition,after the same dose of VLB-PBCA-NP and crude VLB solution were injected into mice tail vein,50% lethal dose of (LD50) was counted.【Results】 The results indicated that there was no significant difference on the effect of rat astrocyte growth between 0.5-50 ng/mL VLB-PBCA-NP and crude VLB solution,but 500 and 5000 ng/mL VLB-PBCA-NP significantly degraded cytotoxicity (P<0.05).5 000 ng/mL VLB-PBCA-NP belonged to toxicity grade 3 on L929 fibroblasts growth,and very significantly degraded crude VLB toxicity.The LD50 of VLB-PBCA-NP and crude VLB solution was respectively 26.38 and 22.7 mg/kg.The LD50 of VLB-PBCA-NP increased by 16.21% compared with crude VLB solution.【Conclusion】 VLB-PBCA-NP degraded acute toxicity and nontumorous cells toxicity of crude VLB. |
| Key words: vinblastine (VLB) nanoparticle L929 cell astrocyte LD50 |
