| 摘要: |
| [目的]针对葡萄酒香气成分气相色谱分析的要求,比较了3种固相微萃取纤维萃取葡萄酒香气成分的效果.[方法]选用常用的PA、PDMS/DVB、PDMS 3种固相微萃取纤维进行葡萄酒香气成分的富集、浓缩处理,然后用GC-MS检测香气成分.通过对不同固相微萃取纤维萃取香气成分的数量、化学种类以及各类化合物累积峰面积标准化值的比较,评价供试固相微萃取纤维萃取葡萄酒香气成分的效果.[结果]3种固相微萃取纤维萃取出的葡萄酒香气成分中,高级醇、化学酯和有机酸是主要成分,萜烯醇、β-大马酮、挥发性酚和呋喃酮等是微量成分.不同固相微萃取纤维萃取的化合物存在明显差异,PA萃取出化合物49种,PDMS萃取出53种,PDMS/DVB仅萃取出38种.累积峰面积标准化值分析表明,PA萃取高级醇、有机酸、3-甲硫基-1-丙醇、2(3H)-二氢呋喃酮、2,4-二季丁基苯酚的灵敏度高;PDMS萃取化学酯类、萜烯醇和β-大马酮的灵敏度高;PDMS/DVB几乎萃取各类化合物的灵敏度都低.[结论]在供试的3种固相微萃取纤维中,PDMS是萃取富集绝大多数葡萄酒挥发性成分的最佳纤维. |
| 关键词: 葡萄酒 香气成分 固相微萃取纤维 气相色谱-质谱联用仪 |
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| 基金项目:国家自然科学基金项目（30571281） |
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| Choosing the best solid phase micro-extraction fiber to extract wine aroma compounds |
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| Abstract: |
| 【Objective】 In order to choose an optimal solid phase micro-extraction fiber to extract wine aroma compounds for GC analysis,three solid fibers often used in wine aroma compounds extraction were tested.【Method】 Solid fibers of PA,PDMS/DVB and PDMS were selected.After extracting,the fiber was immediately inserted into GC injector and volatile compounds were analyzed by GC-MS.The Number of extracted compounds,chemical types of them and the cumulative area normalization value were used to compare extracting effects of these three fibers.【Result】 Results showed that PA,PDMS and PDMS/DVB extracted 49,53 and 38 aroma compounds in the sample wine respectively.Esters,alcohols and organic acids were the majority of the extracted volatile compounds,while terpinols,β-damascenone,volatile phenols and sulphur compounds were the few.The analysis of cumulative area normalization value indicated that PA was more sensitive to absorbing alcohols and organic acids,PDMS more sensitive to esters,PDMS/DVB least sensitivety to alcohols,organic acids and esters.【Conclusion】 All factors above consider,PDMS was the optimal one to extract more aroma compounds in wine for GC analysis among these three fibers. |
| Key words: wine aroma solid phase micro-extraction GC-MS |
