| 摘要: |
| [目的]优化扩展青霉拮抗放线菌CCTCC M207210的液体发酵条件,为其大量生产提供技术支撑.[方法]采用单因素试验和Box-Benhnken试验设计,研究培养温度、初始pH、装液量、接种量对放线菌CCTCC M207210菌体干质量的影响,通过响应面分析优化了各因素综合影响下的培养条件.[结果]放线菌CCTCC M207210的最优培养条件为:接种量135 mL/L,初始pH 7.4,摇床转速180 r/min,培养温度27.6 ℃,250 mL摇瓶装液量125 mL.在此条件下培养72 h,菌体干质量的预测值为6.27 g/L,验证值为6.33 g/L.[结论]在最佳液体培养条件下,CCTCC M207210最大菌体干质量为6.27 g/L,是优化前的2倍. |
| 关键词: 扩展青霉 拮抗放线菌 液体培养 培养条件优化 响应面分析 |
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| 基金项目:国家科技支撑计划项目（2006BAK02A24）；西北农林科技大学青年学术骨干支持计划项目 |
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| Optimization of conditions for liquid cultivation of the antagonistic actinomycete CCTCC M207210 to Penicillium expansum |
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| Abstract: |
| 【Objective】 The paper aimed at optimizing the conditions for liquid cultivation of the antagonistic actinomycete CCTCC M207210 to Penicillium expansum thus providing information for scaling up the cultivation.【Method】 Single factor design and Box Benhnken design were used to select the important factors and their level region.Response Surface Analysis was used to optimize the conditions with complex effect among various factors.【Result】 The optimal conditions were obtained at inoculums’ amount of 135 mL/L,initial pH 7.4,rotation speed 180 r/min,27.6 ℃,and medium volume of 125 mL in 250 mL flask.【Conclusion】 Under the optimal conditions,the dried cell weight of strain CCTCC M207210 reached 6.27 g/L,2 times to the original one. |
| Key words: Penicillium expansum antagonistic actinomycete liquid cultivation optimization of the cultivation condition response surface analysis |
