| 摘要: |
| [目的]对杜仲茎点霉SP-16F发酵生产松脂醇二葡萄糖苷(PDG)的营养条件进行优化,以提高发酵液中的PDG产量.[方法]在单因素实验的基础上,通过三元二次中心组合设计和响应面分析法优化杜仲茎点霉SP-16F发酵产生PDG的最佳营养条件.[结果]在液体培养条件下,茎点霉SP-16F产PDG的最佳培养基组成为:蔗糖47.5 g/L,NaNO3 3.0 g/L,K2HPO4 2.83 g/L.在最佳培养条件下,PDG产量预测值较优化前的最大值提高了3倍以上.[结论]获得了茎点霉SP-16F的最佳产PDG培养基,有效地提高了其发酵产PDG的能力. |
| 关键词: 茎点霉 松脂醇二葡萄糖苷 发酵培养基 营养条件优化 响应面分析 |
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| 基金项目:陕西省科技攻关项目(2008K02-09)；全国大学生科技创新项目(G07036)；西北农林科技大学青年学术骨干支持项目 |
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| Optimization of nutrient elements for the production of pinoresinol diglucoside by a strain of Phoma sp. of Eucommia ulmiodes Oliv.using response surface analysis |
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| Abstract: |
| 【Objective】 The study aimed to optimize the nutrients in the media for the production of pinoresinol diglucoside (PDG) by Phoma sp.SP-16F in submerged cultivation.【Method】 Single factor design and Response Surface Analysis were used to optimize the factors and their levels.【Result】 The optimal nutrients for production of PDG by(PDG) by Phoma sp.SP-16F were obtained as sucrose 47.5 g/L,NaNO3 3.0 g/L,K2HPO4 2.83 g/L.After the optimization,the PDG yield was enhanced by more than 3 times.【Conclusion】 PDG yield was highly improved by optimizing the nutrients for the liquid cultivation of(PDG) by Phoma sp.SP-16F with the aid of Response Surface Analysis. |
| Key words: Phonma sp. Pinoresinol diglucoside fermentation medium optimization of nutrient response surface analysis |
