| 摘要: |
| [目的]研究突触素(SYP)在大鼠动情周期子宫内的分布情况及变化规律.[方法]选择健康、未产、体质量220～250 g成年雌性SD大鼠,采用阴道涂片法鉴定其动情周期,并采用免疫组化超敏SP(Streptavidin-Peroxidase,SP)法研究突触素在大鼠动情周期子宫内分布的变化规律.[结果]大鼠子宫各层组织中均有不同程度SYP免疫阳性产物的分布,并随动情周期的变化表现出一定规律:子宫内膜着色强弱各期差别不大,黏膜上皮细胞、腺上皮细胞及血管内皮细胞基本不着色或少许胞浆淡染,但其基底面均有较深SYP免疫阳性产物分布,动情前期着色最深,动情期、动情后期、动情间期依次减弱,后2期差别不明显;肌层和子宫外膜中,动情前期着色最深,动情期着色减弱,动情后期着色最浅,动情间期表达量明显回升,后3期与动情前期相比差异均极显著(P＜0.01).[结论]突触素在动情周期子宫中的分布表达具有一定的规律,可能受性类固醇激素的调节. |
| 关键词: 突触素 免疫组化SP法 动情周期 子宫 大鼠 |
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| 基金项目:国家自然科学基金项目（39670550）；西北农林科技大学科研专项(06ZR003) |
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| Distribution of synaptophysin in uterus of rats during the estrus cycle |
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| Abstract: |
| 【Objective】 The trial was conducted to study the expression of synaptophysin in uterus of the rats during estrus cycle.【Method】 Sixteen healthy,non-pregnant mature female SD rats of 220 to 250g in body weight were divided randomly into 4 groups of 4 each by vaginal smear,and the expression of synaptophysin in uterus of the rats during estrus cycle was studied by immunohistochemical SP method. 【Result】 The results indicated that synaptophysin-IR products were present in each part of uterus,though the intensity differed,and the distribution of the products in each part of uterus during every period of the estrus cycle was found to adapt to some certain rules:There is no significant difference of the expression of synaptophysin in the endometrium during every period.No or few products in cytoplasm were found in the mucosa epithelium,the glandular epithelium and the blood vessel endothelium.But more products were found to be located in the basal surface of the mucosa epithelium,the glandular epithelium and the blood vessel endothelium.But the density of coloration was the strongest in the preoestrus,and became weaker in the estrum,metoestrus and diestrus,while no significant difference was found during the metoestrus and diestrus.In muscular layer and adventitia,the density of coloration was the strongest in the preoestrus,the significant increase of the products was found in the anoestrum,and the density of the products was a little bit weaker in the estrus than that in the metoestrus.And the differences were all significant (P<0.01).【Conclusion】 The results showed that the expression of synaptophysin in the uterus was found to adapt to some certain rules and might be mediated by the sex steroid hormone. |
| Key words: synaptophysin immunohistochemical estrus cycle uterus rat |
