| 摘要: |
| [目的]探索香桃木叶非精油组分提取物的清除自由基和抗氧化作用,为进一步开发利用香桃木资源提供理论依据和试验资料.[方法]采用水提取法、甲醇-水提取法和乙酸乙酯提取法3种方法从香桃木叶中提取非精油组分,然后用4种化学发光体系和2种比色体系检测其清除自由基和抗氧化作用.[结果]香桃木叶的非精油组分3种提取物均能有效清除O-2、·OH、DPPH·和ONOO-,减轻或消除·OH对DNA的氧化损伤,抑制脂质过氧化,其中甲醇-水提取物清除O-2和ONOO-能力最强,乙酸乙酯提取物清除·OH和DPPH·的效果最佳.[结论]香桃木叶非精油组分是有效的、多功能的天然抗氧化剂和自由基清除剂,具有综合开发利用的价值. |
| 关键词: 香桃木叶提取物 非精油组分 自由基 DNA氧化损伤 抗氧化剂 |
| DOI： |
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| 基金项目:上海市科学委员会资助项目 |
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| In vitro scavenging free radical and antioxidant effects of extracts from Myrtus communis Linn. leaves |
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| Abstract: |
| 【Objective】 The effects of nonessential oil extracts of Myrtus communis Linn.leaves on scavenging free radical and antioxidant role were studied in order to provide an experimental basis for its further development.【Method】 Water,methanol and ethyl acetate extraction method were used to extract non oil components from Myrtus communis Linn.leaves,and then radical scavenging activity and antioxidant role of these extracts were investigated using four chemiluminescence systems and two colorimetric systems.【Result】 Extracts of Myrtus communis Linn.leaves not only effectively scavenged O2,·O H,DPPH· and ONOO-,but reduced or prevented DNA oxidative damage caused by ·OH and inhibited lipided pexoxidation.【Conclusion】 Extracts of Myrtus communis Linn.are effective,multifunctional natural antioxidants and radical scavenger,and are the natural products worthy of further exploitation and utilization. |
| Key words: extract of Myrtus communis Linn.leaf nonessential oil extract free radical DNA oxidative damage antioxidant |
