| 摘要: |
| [目的]探讨达乌里胡枝子黄酮(Lespedeza davurica flavonoids,LDF)的体外抗氧化作用.[方法]分别采用Fenton反应法、邻苯三酚自氧化法和碘-硫代硫酸钠滴定法,测定达乌里胡枝子黄酮对羟自由基、超氧阴离子的清除作用及对猪油、菜油、亚油酸氧化酸败的抑制作用.[结果]达乌里胡枝子黄酮对羟自由基、超氧阴离子有较强的清除作用,在一定范围内,清除效果随质量浓度的升高而加强.当添加的达乌里胡枝子黄酮的质量浓度为1.0 mg/mL时,其对羟自由基的清除作用达到最大,为97.44%;当质量浓度为0.48 mg/mL时,其对超氧阴离子的清除作用最大,可达97.89%;达乌里胡枝子黄酮对猪油、菜油、亚油酸也有极强的抗氧化作用,能够极显著地抑制猪油、菜油过氧化值(POV)的升高(P<0.01)和亚油酸的氧化(P<0.01),这种抑制效果随着达乌里胡枝子黄酮质量浓度的升高而增大.[结论]达乌里胡枝子黄酮具有显著的抗氧化活性,其药用价值值得关注. |
| 关键词: 达乌里胡枝子 黄酮 抗氧化活性 |
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| 基金项目:中央级公益性科研院所(中国农业科学院草原研究所)基本科研业务费专项基金 |
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| Study on the antioxidant activity of flavonoids extracted from Lespedeza davurica |
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| Abstract: |
| 【Objective】 The study discussed the antioxidant activity of Lespedeza davurica flavonoid（LDF）in vitro.【Method】 Fenton reaction, pyrogallic autoxidation and I2Na2S2O3titration were used to determine its scavenging effect on hydroxyl radicals (·OH ) and superoxide anion(O2-) and inhibition on lard,colleseed oil and linoleic acid.【Result】 LDF had good scavenging effect on hydroxyl radicals (·OH) and superoxide anion(O2-),in a certain scope,the effect grew stronger as mass concentration increased.When the mass concentration of additional LDF reached 1.0 mg/mL,the maximum scavenging rate on hydroxyl radicals (·OH) was 97.44%;when the mass concentration was 0.48 mg/mL,the maximum scavenging rate on superoxide anion(O2-) was 97.89%;LDF also had good antioxidation on lard,colleseed oil and linoleic acid.LDF could inhibit POV of lard and collessed oil and inhibit oxidation of linoleic acid significantly (P<0.01),and the effect became stronger as mass concentration of LDF increased.【Conclusion】 The LDF has significant antioxidant activity,and more attention should be paid to its drug value. |
| Key words: Lespedeza davurica flavonoid antioxidant activity |
