| 摘要: |
| [目的]优化棉花黄萎病菌ISSR反应体系,筛选特异性引物,对陕西关中棉花黄萎病菌的分子水平遗传变异及其与地理来源之间的关系进行研究.[方法]用优化好的反应体系和筛选出的引物,对采集自陕西关中6个地区的棉花黄萎病菌和3个参照菌系(新疆和田、陕西泾阳、河南安阳)的亲缘关系进行聚类分析.[结果]优化的反应体系(25 μL)为:dNTP、引物的浓度和Taq DNA聚合酶用量分别为150 μmol/L,0.5 μmol/L,1.5 U.筛选到多态性强的引物842和834.在遗传相似系数为0.36处,将16个菌株分为2个遗传类群.[结论]陕西关中棉花黄萎病菌13个菌株与新疆和田、河南安阳菌系的亲缘关系较远,与泾阳菌系的亲缘关系较近,且来源于同一地域的菌株亲缘关系较近. |
| 关键词: 棉花黄萎病菌 ISSR标记 反应体系优化 聚类分析 遗传多样性 |
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| 基金项目:欧盟科技合作项目 |
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| ISSR Analysis of Verticillium Dahliae of Cotton in Shaanxi Guanzhong Area |
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| Abstract: |
| 【Objective】 The ISSR-PCR reaction system was optimized.The primers which produced clear and rich polymorphism bands were screened.The genetic similarity and their sources of Verticillium dahliae from Shaanxi were tested.【Method】 13 isolated V.dahliae strains from Shaanxi of V.dahliae and 3 standard strains (from Xinjiang hetian,Shaanxi jingyang and Henan anyang) were tested so as to find out the correlation between genetic diversity of Verticillium dahliae. 【Result】 The optimal concentration reaction system was 0.5 μmol/L primer,1.5 U-Taq-DNA polymerase,150 μmol/L dNTP.Two primers 842 and 834 were screened.The isolated Verticillium dahliae strains were clustered into 2 genetic line ages at 0.36 genetic similarity.【Conclusion】 The strains from Shaanxi have a distant relationship with V.dahliae strains from Xinjiang and Anyang,and shows some relation with their geographical originals. |
| Key words: Verticillium dahliae ISSR marker reaction system optimization cluster analysis genetic diversity |
