| 摘要: |
| [目的]筛选与蛋品质紧密连锁的微卫星标记,为蛋品质分子辅助育种提供参考.[方法]应用20对微卫星标记,对40周龄文昌产蛋母鸡基因座位进行多样性检测,并对每个个体所产鸡蛋的品质(哈氏单位、蛋质量、蛋壳强度)进行测定,通过相关分析,筛选与哈氏单位、蛋质量、蛋壳强度相关的分子遗传标记.[结果]20个微卫星座位的平均多态信息含量为0.617,杂合度为0.190～0.784,各位点的杂合度均较高,其中最高的是LEI0086,最低的是LEI0229.影响40周龄文昌鸡哈氏单位的标记有2号染色体上的标记MCW0239和4号染色体上的标记ADL0260;2号染色体上的标记LEI0086与40周龄文昌鸡蛋质量有关;Z染色体上的标记LEI0121与40周龄文昌鸡蛋壳强度有关.[结论]研究结果为文昌鸡蛋品质的间接选择提供了理论依据. |
| 关键词: 文昌鸡 微卫星标记 基因多态性 鸡蛋品质 相关性 |
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| 基金项目:国家科技基础条件平台项目，农业部农业科技跨越计划 |
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| Study on the relationship between microsatellite markers and egg quality traits in Wenchang chicken |
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| Abstract: |
| 【Objective】 The research was to try to find microsatellite markers related to egg quality and make preparation for MAS on egg quality. 【Method】 20 microsatellite markers were selected to detect the gene loci of 40w Wenchang chicken breeds.The eight egg traits (including egg weight,shell weight,shell thickness,shell strength,haugh unit,egg shape index,yolk weight,yolk color ) were tested in 140 Wenchang chicken individuals. The relationship between microsatellite loci and Haugh unit,egg weight,shell strength was screened by correlation analysis. 【Result】 The results showed that the mean polymorphism information content (PIC) was 0.617 and the mean heterozygosity (H)0.190-0.784 in 20 microsatellite sites.The highest H was in LEI0086 and the lowest in LEI0229.The markers related to Haugh unit (HU) at 40 weeks were MCW0239 on Chromosome 2 and MCW0260 on Chromosome 4.LEI0086 on Chromosome 2 had an effect on egg weight (EW) and the markers related to shell strength (SS) at 40 weeks was LEI0121 on Chromosome Z. 【Conclusion】 These results could provide basic molecular data and scientific basis for indirect choice of egg quality. |
| Key words: Wenchang chicken microsatellite marker gene variability chicken egg quality relationship |
