| 摘要: |
| [目的]检测致病性大肠埃希氏菌中超广谱β-内酰胺酶(ESBLs)的基因型,初步研究β-内酰胺酶抑制剂(BLI)-他唑巴坦的抑酶保护作用.[方法]对产生ESBLs的5株致病性大肠埃希氏菌,设计并合成TEM-1和是SHV-1 2对引物,用PCR方法进行了ESBLs的DNA扩增、测序和基因型分析;用试管二倍稀释法测定头孢噻呋与BLI-他唑巴坦钠以不同配比对产生ESBLs大肠埃希氏菌的最小抑菌浓度.[结果]从产生ESBLs细菌中均扩增出了TEM型ESBLs产物,但未扩增出SHV型ESBLs产物;头孢噻呋与BLI-他唑巴坦钠(质量比1∶1～8∶1)联合使用,对产生ESBLs的大肠埃希氏菌的最小抑菌浓度较头孢噻呋单独使用基本降低,最高可降低16倍.[结论]国内畜禽产生ESBLs的大肠埃希氏菌未通过质粒进行细菌间耐药基因的交换, ESBLs的产生与生物种属没有关系,BLI-他唑巴坦有较强的抑制ESBLs作用. |
| 关键词: β-内酰胺酶 BLI-他唑巴坦 大肠埃希氏菌 耐药性 基因型 |
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| 基金项目:河南省教育厅资助项目，河南省科技厅资助项目 |
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| Genotype detection of extend spectrum β-lactamase of pathogenic Escherichia coli and the inhibitive effect of BLI- tazobactam |
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| Abstract: |
| 【Objective】 The study was to detect genotype of extend spectrumβ-lactamase in pathogenic Enterobacteriaceaes，and to study the inhibitive effect of Tazobactam，a β-lactamases inhibitor(BLI).【Method】 For five Enterobacteriaceaes tested for ESBLs by double-disk method,two primers were designed and synthetized:TEM-1and SHV-1,ESBLs gene type was amplified,sequenced and analyzed by PCR;the minimal inhibitory concentrations(MICs) of Cetiofur combined BLI-Tazobactam to the five Enterobacteriaceaes according to different proportions were carried out with two fold dilution method. 【Result】 The producd bacteria ESBLs were amplified TEM type ESBLs fully,but no SHV type ESBLs;the minimal inhibitory concentrations(MICs) of BLI Tazobactam combined Cetiofur according to different mass ratios （1∶1-8∶1）to the ESBLs bacteria reduced sixteen times compared with Cetiofur. 【Conclusion】 ①ESBLs bacteria have not transmited resistant gene through plasmids;②The production of ESBLs has no relation with animal genus;③The BLI-Tazobactam has better activity to inhibit ESBLs. |
| Key words: β-lactamase BLI-Tazobactam Enterobacteriaceae resistance gene |
