| 摘要: |
| [目的]获得抑菌活性更强的小果博落回的生物碱成分.[方法]采用4种亲核性溶剂(甲醇、乙醇、丙酮和饱和Na2CO3碱溶液)和1种非亲核性溶剂(四氢呋喃),分别从吸附了小果博落回总生物碱的D101树脂上洗脱生物碱,用薄层层析法(TLC)进行成分分析,以金黄色葡萄球菌、无乳链球菌、大肠杆菌、肠形点状产气单胞菌、嗜水气单胞菌、巴氏杆菌、蜡样芽孢杆菌和鳗弧菌等8种菌株为供试菌,采用药敏纸片法进行抑菌活性测定,研究小果博落回在不同溶剂处理下所得的总生物碱成分和抑菌活性的变化.[结果]TLC分析结果表明,经过乙醇、甲醇、碱溶液和丙酮作用后,所得总生物碱(分别为SEtOH、SMeOH、Sbase和Sacetone)与直接用酸提取的总生物碱(Saw)在成分上存在差异;5种总生物碱均具有抑菌活性,其抑菌活性依次表现为SMeOH>SEtOH≈Sacetone≈Sbase>Saw.[结论]提取过程中使用亲核性溶剂,可引起小果博落回总生物碱成分的变化和抑菌活性的改变,其中以甲醇提取的总生物碱的抑菌活性较强. |
| 关键词: 小果博落回 生物碱 抑菌活性 |
| DOI： |
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| 基金项目:国家自然科学基金 |
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| Antibacterial activity of the total alkaloids from Macleaya microcarpa |
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| Abstract: |
| 【Objective】 The study was to obtain stronger anti-bacterial alkaloids activity,and the changes of both the components and the antibacterial activity of the different total alkaloids (TAs) from Macleaya microcarpa dealt with different solvents were studied.【Method】 The different TAs were obtained from the same TAs absorbed on D101 macroporous resin eluted by four different nucleophilic solvents(methanol,ethanol,acetone and saturated Na2CO3 aqueous solution) and one nonucleophilic solvent (tetrahydrofuran). The components of all TAs were analyzed by thin layer chromatography (TLC). The antibacterial activity of the TAs was assayed by drug sensitive slips method in vitro against Staphylococcus aureas,Streptococcus agalactiae,Escherichia coli,Aeromonas Punctat fintestinalis,Aeromonas hydrophila,Pasteurella multocida,Bacillus cereus and Vibrio anguillarum.【Result】 The result showed that there was a difference between the components of the TAs extracted by acid solution (Saw) and those of the ones reacted with ethanol (SEtOH), methanol (SMeOH),alkali solution (Sbase) and acetone (Sacetone),respectively.All the five TAs showed the antibacterial activity against all the tested bacteria, and the order of the activity was SMeOH>SEtOH≈Sacetone≈Sbase>Saw.【Conclusion】 Using the nucleophilic solvents in the extraction process, the components and the antibacterial activity of the total alkaloids of Macleaya microcarpa were changed.And the TAs extracted by methanol possessed stronger anti bacterial activity. |
| Key words: Macleaya microcarpa alkaloid antibacterial activity |
