| 摘要: |
| [目的]明确放线菌19G-317菌株发酵产物对植物病原菌的抑制活性,为该菌株在农业病害防治中的开发应用及后续研究奠定基础.[方法]采用生长速率法、孢子萌发法、活体组织法和盆栽试验,测定放线菌19G-317菌株发酵产物的抑菌效果. [结果]生长速率法试验表明,放线菌19G-317菌株发酵产物对供试22种植物病原菌菌丝生长均有不同程度的抑制作用,发酵液对其中11 种植物病原菌的抑制率大于60%,菌丝提取物对其中8 种植物病原菌的抑制率大于90%;发酵液和菌丝提取物对油菜菌核病菌菌丝生长的EC50分别为84.30和18.80 mg/L.孢子萌发试验表明,发酵液和菌丝提取物对番茄灰霉病菌孢子的萌发均有较强的抑制作用,在0.5 mg/mL质量浓度下,其抑制率分别为94.26%和96.20%;组织法试验表明,在10 mg/mL质量浓度下,发酵液和菌丝提取物对番茄灰霉病的药效分别为72.17%和76.95%;盆栽试验表明,19G-317菌株发酵液和菌丝提取物对辣椒疫霉病均有一定的保护和治疗作用.[结论]放线菌19G-317菌株抑菌谱较广,对植物病菌的防治有很大的研究开发潜力. |
| 关键词: 放线菌19G-317菌株 发酵产物 抑菌活性 |
| DOI： |
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| 基金项目:陕西省13115科技创新工程项目 |
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| Antifungal activity of actinomycete strain 19G-317 |
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| Abstract: |
| 【Objective】 The research primarily studied the antifungal activity of the fermentation product of actinomycete strain 19G-317.【Method】 The antifungal activity of the fermentation product of actinomycete strain 19G-317 was researched by mycelium growth rate,spore germination test,tissue culture method and potted plant method.【Result】 The results of mycelium growth rate test showed that the fermentation product had different inhibition effects on 22 tested plant pathogenic fungi.The inhibition rate of the fermentation broth was over 60% to 11 tested plant pathogenic fungi,and that of the extract product of mycelium was over 90% to 8 tested plant pathogenic fungi.The EC50 values of the fermentation broth and the extract product of mycelium against Sclerotinia sclerotiorum were 84.30 mg/L and 18.80 mg/L respectively.The results of spore germination test showed that the inhabition rates of the fermentation broth and extract product of mycelium were 94.26% and 96.20% to Botrytis cinerea under the concentration of 0.5 mg/mL respectively.Tissue culture method tests showed that the inhabition rates of the fermentation broth and the extract product of mycelium were 72.17% and 76.95% to Botrytis cinerea under the concentration of 10 mg/mL respectively.The results of potted test indicated that the fermentation products had both protective and therapeutic efficacy on P.capsici.【Conclusion】 These studies indicate that a potential exists for utilizing 19G-317 to control plant diseases. |
| Key words: actinomycetes strain 19G-317 fermentation product antifungal activity |
