| 摘要: |
| [目的]探讨西瓜苗期炭疽病抗性与叶片组织中过氧化物酶(POD)、多酚氧化酶(PPO)活性及叶绿素、可溶性蛋白含量的关系.[方法]以抗性不同的西瓜品种西农八号、M08(抗病品种)和红小玉、玲珑王(感病品种)为材料,在苗期用1×106mL-1炭疽病菌孢子悬浮液接种,于接种当天(接种前)及接种后第2,4,6,8天,对西瓜叶片中与抗性有关的POD、PPO活性及叶绿素,可溶性蛋白含量进行了测定.[结果]西瓜炭疽病抗性与叶片组织中POD、PPO活性以及叶绿紊、可溶性蛋白含量密切相关.接种炭疽病菌后,各品种西瓜叶片组织中POD和PPO活性都显著提高,抗病品种POD和PPO活性始终高于感病品种;叶绿素含量与品种的抗病性呈正相关;接种后各西瓜品种叶片可溶性蛋白含量均高于对照,但感病品种峰值出现时间较抗病品种早.[结论]不同抗性西瓜品种对炭疽病的抗性与POD、PPO活性及叶绿素含量存在一定的正相关,与可溶性蛋白含量存在一定的负相关. |
| 关键词: 西瓜 炭疽病菌 生理生化变化 苗期抗性 |
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| 基金项目:国家科技支撑计划? |
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| Biochemical and physiological changes of different watermelon cultivars inoculated by Colletotrichum orbiculare |
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| Abstract: |
| 【Objective】 The study determined the relationship between watermelon cultivars of anthracnose resistance and POD,PPO and the content of chlorophyll and soluble protein.【Method】 The activity of peroxidase (POD),polyphenol oxidase (PPO) and the content of chlorophyll and soluble protein in the leaves of four watermelon cultivars with different resistance to Colletotrichum orbiculare were measured.Xinongbahao and Hongxiaoyu were resistant cultivars,Hongxiaoyu and Linglongwang were susceptible cultivars.The seedlings inoculated by 1×106 mL-1 spore solution were studied before inoculation and on the 2nd,4th,6thand 8th day.【Result】 The results showed that there was a close relation between watermelon cultivars of anthracnose resistance and POD,PPO and the content of chlorophyll and soluble protein.POD and PPO activities were all enhanced obviously after inoculation, and the activities of resistant cultivars were higher than those of the susceptible cultivars.The content of chlorophyll and the resistance of cultivar had positive relativity.The contents of soluble protein of the inoculated were all higher than the comparison,but the peak value of the cultivars whose resistance appeared earlier than the susceptible cultivars.【Conclusion】 The relative POD and PPO activity and chlorophyll content were possibly correlated with disease resistance of the tested watermelon cultivars.Negative relationship was possibly in existence between the content of soluble protein and the disease resistance of the tested watermelon cultivar. |
| Key words: watermelon Colletotrichum orbiculare biochemical and physiological changes seedling anthrachos resistance |
