| 摘要: |
| [目的]探讨脱氧鬼臼毒素对粘虫生殖力的影响.[方法]以小叶碟添加法处理粘虫幼虫,测定脱氧鬼臼毒素对其生长发育的影响.采用混药饲喂法测定脱氧鬼臼毒素对成虫生殖力相关指标的影响.[结果]以0.1,0.3和0.5 mg/mL脱氧鬼臼毒素处理的叶碟饲喂粘虫5龄幼虫后,其化蛹率、羽化率、单雌产卵量和孵化率均明显下降,且异常蛹率升高;以含0.3和0.5 mg/mL脱氧鬼臼毒素的药蜜分别或同时饲喂粘虫雌、雄蛾后,均可致产卵前期延长、产卵期和寿命缩短,产卵量、孵化率和幼虫存活率下降;以含0.3 mg/mL脱氧鬼臼毒素的药蜜饲喂粘虫雌蛾后,卵巢管长度和卵巢鲜质量分别降低了27.36%和41.48%,腹部脂肪含量下降了10.45%,卵巢内DNA、RNA和可溶性蛋白含量分别下降了18.93%,13.24%和16.39%.[结论]脱氧鬼臼毒素对粘虫的生殖力具有明显抑制作用. |
| 关键词: 脱氧鬼臼毒素 粘虫 生殖力 生长发育 卵巢发育 |
| DOI： |
| 分类号:Q965.9 S482.39 |
| 基金项目:国家自然科学基金? |
|
| Effect of deoxypodophyllotoxin on fecundity of Mythimna separta Walker |
|
WANG Jian-bin MA Zhi-qing FENG Jun-tao ZHANG Xing
|
| Abstract: |
| 【Objective】 In order to study the effect of deoxypodophyllotoxin on the reproduction of Mythimna separta Walker,the development of the larvae and the related fecundity indexes of the adult of Mythimna separta W.were assayed.【Method】 The method of disc leaf was appended to test the effect of deoxypodophyllotoxin on the development of the larvae and the way of feeding by containing deoxypodophyllotoxin of honey to assay the related fecundity indexes of the adult of Mythimna separta W.【Result】 The results showed that the rate of pupation,eclosion and the quantity of oviposition and the rate of egg hatching decreased significantly at the concentration of 0.1,0.3 and 0.5 mg/mL.At the same time,the rate of the abnormal pupae increased after 5th instar treatment.The period of preoviposition was prolonged and the period of oviposition and life were shortened.Furthermore,the number of oviposition,the rate of hatching and the rate of larvae survival decreased after feeding 0.3 and 0.5 mg/mL deoxypodophyllotoxin to the adult of male and female at the same time or separately.The length of ovary tubes and wet weight of ovaries decreased 27.36% and 41.48%.The content of female fat and DNA and RNA and soluble protein in ovary decreased 10.45%,18.93%,13.24% and 16.39% at 0.3 mg/mL respectively.【Conclusion】 Therefore,the deoxypodophyllotoxin has apparent effect on fecundity of Mythimna separta W. |
| Key words: deoxypodophyllotoxin Mythimna separta W. fecundity development overy development |
