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| 抗球虫药对球虫病雏鸡部分血清生化指标的影响 |
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李蕴玉1, 李佩国1, 张香斋1
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河北科技师范学院 动物科学系
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| 摘要: |
| [目的]探讨中草药和球敌对雏鸡人工感染柔嫩艾美耳球虫(E.tenella)后部分血清生化指标的影响.[方法]将160只14日龄海兰褐雏鸡随机均分为对照组(I)、感染组(Ⅱ)、中草药组(Ⅲ)和球敌组(Ⅳ).除I组外,其余组每只鸡口服感染8.0×104个E.tenella孢子化卵囊,并且Ⅲ、Ⅳ组鸡于感染当天分别饮用含生药10 g/L的抗球虫中草药饮水剂和有效质量浓度为1 mg/L的球敌,连用12 d.分别于感染前和感染后3,6,9,12 d,各组随机抽取5只鸡,心脏采血,分离血清,测定血清总蛋白(TP)、白蛋白(ALB)、甘油三酯(Tg)、尿酸(UA)、肌酐(Cre)含量和乳酸脱氢酶(LDH)、碱性磷酸酶(ALP)活性.[结果]与Ⅱ组比较,Ⅲ、Ⅳ组TP含量在感染后6,9 d分别提高了22.31%(P＜0.05),33.57%(P＜0.05)和17.48%(P＜0.05),24.23%(P＜0.05);在感染后6 d,ALB含量提高了26.17%(P＞0.05)和48.23%(P＜0.05),Tg含量提高了49.25%(P＜0.01)和40.30%(P＜0.01);UA含量在感染后3 d降低了42.39%(P＜0.01)和42.48%(P＜0.01);LDH活性在感染后3,6 d分别降低了30.77%(P＜0.01),30.13%(P＜0.01)和23.36%(P＜0.05),21.21(P＜0.05).Ⅲ、Ⅳ组血清Cre含量及ALP活性与Ⅱ组相比差异不显著,但在感染后6 d,分别降低了17.51%,12.52%和16.92%,14.39%.与I组相比,在感染后3 d,Ⅲ组血清TP含量提高了24.94%(P＜0.05),而Ⅳ组Tg含量降低了32.99%(P＜0.05);Ⅲ、Ⅳ组UA含量在感染后3,6,9 d分别提高了111.66%(P＜0.01),111.36%(P＜0.01);69.45%(P＜0.01),76.25(P＜0.01)和35.09%(P＜0.05),43.99%(P＜0.05);Cre含量在感染后3,6 d分别提高了45.41%(P＜0.05),49.94%(P＜0.05)和49.97%(P＜0.05),59.05%(P＜0.05),而其他指标的差异不显著.[结论]中草药和球敌通过抵抗球虫感染,在一定程度上有助于保持血清生化指标的相对恒定. |
| 关键词: 鸡球虫病 中草药 球敌 血清生化指标 |
| DOI： |
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| 基金项目:河北省畜牧兽医局资助项目（2003-12） |
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| Effects of anticoccidial drugs on some biochemical indexes in sera of chickens with coccidiosis |
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| Abstract: |
| 【Objective】 The effects of traditional Chinese medicine and qiudi on some biochemical indexes in sera of chickens infected with E.tenella were studied. 【Method】 One hundred and sixty hyline brown chickens of 14-day-old were randomly and equally divided into control group(Ⅰ),infected group(Ⅱ),traditional Chinese medicine group (Ⅲ）and qiudi group(Ⅳ).All chickens were orally inoculated with 8×104sporulated oocysts of Eimeria tenella except chickens of groupⅠ,at the same time the chicks of group Ⅲ and Ⅳ received water with 10 g/L（crude drug）of anticoccidial herbal medicine and 1 mg/L（effective concentration）of qiudi for 12 days respectively.Five chicks were selected at random from each group and bled from hearts at pre infection and 3,6,9,12 day post-infection,then sera were separated .The contents of TP，ALB，Tg，UA，Cre and activities of LDH，ALP were measured.【Result】 Compared with groupⅡ,seral TP content of group Ⅲ and group Ⅳ significantly increased by 22.31%（P＜0.05）,33.57%（P＜0.05） and 17.48%（P＜0.05）,24.23%（P＜0.05）respectively on the 6th,9th day post infection(dpi),the contents of ALB and Tg also increased by 26.17%（P＞0.05), 48.23%（P＜0.05）and 49.25%（P＜0.01）,40.30%（P＜0.01）on 6 dpi, but UA content significantly decreased by 42.39%（P＜0.01）and 42.48%（P＜0.01）on 3 dpi,the activity of LDH significantly decreased by 30.77%（P＜0.01）,30.13%（P＜0.01）and 23.36%（P＜0.05）,21.21%（P＜0.05）on 3 dpi and 6 dpi,the content of Cre and ALP activity decreased by 17.51%,12.52% and 16.92% ,14.39% respectively,on the 6th day of post-infection.Compared with group Ⅰ,seral TP content of group Ⅲ significantly increased by 24.94%（P＜0.05）on 3 dpi,but Tg content of group Ⅳdecreased by 32.99%（P＜0.05）， and UA content of group Ⅲ and Ⅳ significantly increased by 111.66%（P＜0.01）,111.36%（P＜0.01）;69.45%（P＜0.01）,76.25%（P＜0.01） and 35.09%（P＜0.05）,43.99%（P＜0.05）respectively on 3 dpi,6 dpi and 9 dpi,the content of Cre significantly increased by 45.41%（P＜0.05）,49.94（P＜0.05）and 49.97%（P＜0.05）,59.05%（P＜0.05） on 3 dpi and 6 dpi,but the other parameters were not significant.【Conclusion】 The results implied that traditional Chinese medicine and qiudi could keep the relative stability of seral biochemical indexes by resisting coccidial infection to a certain extent. |
| Key words: chicken coccidiosis traditional Chinese medicine qiudi seral biochemical index |
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