| 摘要: |
| [目的]初步探讨了日粮镁调控肉仔鸡肝脏中过氧化氢酶(catalase,CAT)活性的途径.[方法]将128只AA肉仔鸡随机均分到对照组和加镁日粮组,对照组日粮为普通玉米-豆粕日粮,在此基础上每kg日粮添加10 g天门冬氨酸镁(MgAsp)作为加镁日粮组,研究日粮镁对肉仔鸡肝脏CAT活性的影响.[结果]与对照组相比,加镁日粮组肉仔鸡肝细胞中总镁和肝细胞内游离态镁浓度分别提高了23.8%(P<0.01)和19.4%(P<0.01);肝脏CAT活性及其mRNA水平分别提高了14.3%和21.4%(P<0.01);肝细胞总镁、肝细胞内游离态镁与CAT活性、CAT基因mRNA丰度间均存在显著正相关(P<0.05).[结论]日粮中添加镁提高了肉仔鸡肝细胞内游离态镁浓度,增强了CAT基因表达,从而提高了CAT活性. |
| 关键词: 镁 游离态镁 过氧化氢酶 基因表达 肉仔鸡 |
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| 基金项目:国家自然科学基金项目(30371043) ；郑州牧业工程高等专科学校博士基金项目（200602） |
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| A pilot study on the pathways of dietary magnesium modulates on catalase activity in the liver of broiler chickens |
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| Abstract: |
| 【Objective】 The object of the present work was to investigate the pathways of dietary magnesium modulates catalase (CAT) activity in the liver of broiler chickens.【Method】 One hundred twenty eight male Abor Acre broiler chickens were randomly allocated into two groups.Maize and soybean meals were the main ingredients of the control diet,and the control diet supplemented with 10 g/kg MgAsp served as the Mg supplementation diet.【Result】 Compared with the control,hepatic total Mg and ionized Mg concentrations in hepatocytes increased by 23.8% and 19.4% respectively (P<0.01),while the hepatic CAT activity and its gene mRNA level increased by 14.3% and 21.4% respectively (P<0.01).Positive correlations among hepatic total magnesium (ionized Mg in hepatocytes),CAT activity and its gene mRNA were observed.【Conclusion】 The results indicated that dietary Mg supplementation increasesd ionized Mg concentration in hepatocytes and promoted CAT gene expression,and consequently CAT activity was enhanced. |
| Key words: magnesium ionized Mg catalase gene expression broiler chicken |
