| 摘要: |
| [目的]评价纳豆芽孢杆菌对瘤胃液体外发酵的影响.[方法]采用瘤胃液体外发酵产气法,以不添加纳豆芽孢杆菌为对照,动态观测基础日粮中纳豆芽孢杆菌添加量分别为1.5×108/g(处理Ⅰ)和7.5×108/g(处理Ⅱ)对瘤胃液体外发酵产气量、pH值、N3-N质量浓度和各挥发性脂肪酸浓度的影响.[结果]与对照相比,处理Ⅱ产气量提高了11.89%(P<0.05),瘤胃pH值显著降低(P<0.05),NH3-N质量浓度提高了16.07%(P<0.05),乙酸、丙酸和总挥发性脂肪酸浓度分别提高了10.43%,50.35%和20.70%(P<0.05);处理Ⅰ除丙酸浓度显著高于对照(P<0.05)外,其他各指标均与对照无明显差异(P>0.05).[结论]纳豆芽孢杆菌培养物在基础日粮中的添加量为7.5×108/g时,能促进瘤胃液对饲料底物的降解代谢. |
| 关键词: 纳豆芽孢杆菌 产气法 瘤胃液体外发酵 |
| DOI： |
| 分类号: |
| 基金项目:科技部国际科技合作重点项目（2004DFA06700） |
|
| Assessment of effect on supplementation of Bacillus subtilis natto on ruminal fermentation in vitro by gas production technique |
|
|
| Abstract: |
| 【Objective】 The study is to evaluate the role of Bacillus subtilis natto culture (BNC) effect on ruminal fermentation in vitro.【Method】 Gas production technique was used to examine the effect of supplementation of 0.15% concentration BNC in dry material (DM) of substrate,containing 1.5×108/g（groupⅠ）and 0.75% concentration BNC in DM of substrate,containing 7.5×108/g (groupⅡ) on ruminal gas production,pH value,NH3-N concentration and VFA concentrations in vitro.【Result】 Compared with control group (CK),the gas production in treat groupⅡ increased by 11.89% (P<0.05),the pH value decreased (P<0.05),the NH3-N concentration increased by 16.07% (P<0.05),and the concentrations of acetic acid, propionic acid and total VFA increased by 10.43%,50.35% and 20.70% (P<0.05) respectively.In groupⅠ,propionic acid concentration was higher than that of CK,and the other indicators were not significantly different with CK.【Conclusion】 The BNC containing 7.5×108/g in DM of substrate promoted the degradation and metabolism of the substrate in ruminal fermentation in vitro. |
| Key words: Bacillus subtilis natto gas production technique ruminal fermentation in vitro |
