| 摘要: |
| [目的]克隆棉蚜HSP70基因cDNA片段,并分析其基因序列.[方法] 采集黄色型和绿色型棉蚜,实验室饲养4～5代形成单克隆系,温度处理后提取总RNA备用.根据已知昆虫HSP70基因的保守性区域,设计特异性引物,采用RT-PCR方法扩增棉蚜HSP70基因的部分序列,测序后进行核苷酸和推导的氨基酸序列分析.[结果] RT-PCR扩增得到一条长度783 bp左右的HSP70基因cDNA片段.测序结果显示,所得cDNA片段长度为783 bp(GenBank登录号为:EU109426),且黄色型和绿色型棉蚜的核苷酸序列一致,编码261个氨基酸残基.由棉蚜该片段推导的氨基酸序列与其他昆虫的同源性较高.[结论] 获得了棉蚜HSP70基因783 bp的cDNA片段,该片段编码261个氨基酸残基. |
| 关键词: 棉蚜 克隆 热休克蛋白70 基因 同源性 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金项目（39970112，30470268）；陕西省重点项目（2001SM01） |
|
| Clone and sequence analysis of cDNA fragment of heat shock protein 70 gene in the aphis gossypii Glover |
|
|
| Abstract: |
| 【Objective】 The study cloned the partial cDNA sequences of HSP70 genes of Aphis gossypii Glover and studied the features of the sequences.【Method】 Cotton aphids of yellow and green type were collected laboratory and reared for 4-5 generations.After temperature treatment total RNA was extracted to reserve.According to the conserved regions of other known insects,a pair of specific primers were designed and the RT-PCR method was used to amplify the partial sequences of HSP70 from cotton aphid,then analyzed the nucleotide and deduced amino acid sequence.【Result】 Sequencing results show that the cDNA fragment was 783 bp in size(GenBank accession No.EU109426).The nucleotide sequence of the yellow type and green type were the same.It encoded 261 amino acid residues.There was high homology of amino acid sequence between the cotton aphid and other insects.【Conclusion】 Sequencing result show that the cDNA fragment was 783 bp in size,it encoded 261 amino acid residues. |
| Key words: Aphis gossypii Glover clone heat shock protein 70 gene homology |
